Document Detail


Production of dextran from sucrose by a newly isolated strain of Leuconostoc mesenteroides (PCSIR-3) with reference to L. mesenteroides NRRL B-512F.
MedLine Citation:
PMID:  11592914     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A newly isolated strain of Leuconostoc mesenteroides (PCSIR-3) produced a different dextran compared with that of L. mesenteroides NRRL B-512F. Different media compositions used for dextran production showed that media containing CaCl(2) produced dextran in higher quantities compared with other media. The viscosity of the dextran produced in different media varied in nature. Dextran from media 1 and 2 was of higher molecular mass compared with that from media 3, 4 and 5. Dextran production is also effected by the sucrose concentration in the media. The higher the initial concentration of sucrose, the higher is the yield of dextran produced per unit volume; however, the percentage conversion of sucrose into dextran decreases. A continuous drop in pH was associated with growth and dextran production. The yield of dextran increases during the growth phase and maximum yield was obtained at the end of the exponential phase. Dextran produced by L. mesenteroides PCSIR-3 is quite different from the dextran produced by NRRL B-512F. Maximum dextran production from L. mesenteroides PCSIR-3 occurs in 18 h compared with 12 h for NRRL B-512F.
Authors:
S A Ul-Qader; L Iqbal; H A Rizvi; R Zuberi
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Biotechnology and applied biochemistry     Volume:  34     ISSN:  0885-4513     ISO Abbreviation:  Biotechnol. Appl. Biochem.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-10-10     Completed Date:  2002-01-23     Revised Date:  2007-03-21    
Medline Journal Info:
Nlm Unique ID:  8609465     Medline TA:  Biotechnol Appl Biochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  93-7     Citation Subset:  IM    
Affiliation:
Pharma & Fine Chemical Research Centre, P.C.S.I.R. Laboratories Complex, Karachi, Pakistan. madar01@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / metabolism
Dextrans / biosynthesis*,  metabolism
Fermentation
Hydrogen-Ion Concentration
Leuconostoc / enzymology,  growth & development,  isolation & purification*,  metabolism*
Reference Standards
Species Specificity
Sucrase / metabolism
Sucrose / metabolism*
Time Factors
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 57-50-1/Sucrose; 9004-54-0/Dextrans; EC 3.2.1.48/Sucrase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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