| Production and derivate composition of trisporoids in extended fermentation of Blakeslea trispora. | |
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MedLine Citation:
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PMID: 20596706 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Trisporic acid, its precursors and derivatives are used within zygomycete fungi as communication signals and sexual regulators, and also influence the production rate of the parent compound, beta-carotene. Cultivation parameters during growth and the trisporoid production phase of Blakeslea trispora were studied in two-step shake flask cultures and up-scaled fermentations. Comparison of various fermentation protocols allowed the definition of parameters governing trisporoid production. Highest yields were obtained when the initial growth phase allowed for both rapid growth and fast exhaustion of nitrogen and phosporous sources. Onset of trisporoid production is accompanied by a pH drop in the medium and triggered by nutrient limitation, nitrogen depletion being the most important factor. Supplementation of cultures with carbon at low concentration after onset of trisporoid production led to prolonged growth and higher final product accumulation. B. trispora produces trisporoids in two major series, B and C. During a first peak in trisporic acid accumulation, production of trisporic acid B exceeds that of trisporic acid C, which later accumulates at the expense of the trisporic acid B, indicating a variable regulation of the ratio between these metabolites. These data are valuable for tailoring production systems for enrichment of specific intermediates of this complex signal family. |
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Authors:
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Doreen Schachtschabel; Klaus-Dieter Menzel; Gudrun Krauter; Anja David; Martin Roth; Uwe Horn; Wilhelm Boland; Johannes Wöstemeyer; Christine Schimek |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-02 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 88 ISSN: 1432-0614 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-16 Completed Date: 2010-11-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 241-9 Citation Subset: IM |
Affiliation:
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Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, 07745, Jena, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carbon
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metabolism Culture Media / chemistry Fatty Acids, Unsaturated / chemistry, metabolism* Fermentation Hydrogen-Ion Concentration Mucorales / growth & development, metabolism* Nitrogen / metabolism Phosphorus / metabolism beta Carotene / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Culture Media; 0/Fatty Acids, Unsaturated; 11043-91-7/trisporic acid; 7235-40-7/beta Carotene; 7440-44-0/Carbon; 7723-14-0/Phosphorus; 7727-37-9/Nitrogen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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