| Production of delta-aminolevulinic acid: characterization of murine liver 4,5-dioxovaleric acid: L-alanine aminotransferase. | |
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MedLine Citation:
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PMID: 1773884 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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1. We report on the kinetic properties of murine liver 4,5-dioxovaleric acid:L-alanine aminotransferase (DOVA transaminase). 2. The transamination of 4,5-dioxovaleric acid (DOVA) led to the production of delta-aminolevulinic acid. 3. L-Alanine was the preferred amino group donor among the common 20 amino acids. 4. The optimum pH of the reaction was 7-8. 5. A Km of 220 microM for DOVA and a Km of 970 microM for L-alanine were obtained. 6. The reaction was inhibited by each of the following: glyoxylate, beta-chloroalanine, methylglyoxal, delta-aminolevulinate, pyruvate, heme, and gabaculine. 7. None of several xenobiotic inducers of microsomal mixed function oxidases tested had a significant effect on DOVA transaminase activity in studies performed with murine primary hepatocyte cultures. |
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Authors:
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C E McKinney; I Z Ades |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The International journal of biochemistry Volume: 23 ISSN: 0020-711X ISO Abbreviation: Int. J. Biochem. Publication Date: 1991 |
Date Detail:
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Created Date: 1992-03-03 Completed Date: 1992-03-03 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0250365 Medline TA: Int J Biochem Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 803-10 Citation Subset: IM |
Affiliation:
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Department of Zoology, University of Maryland, College Park 20742. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aminolevulinic Acid
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metabolism* Animals Cell Fractionation Cells, Cultured Kinetics Male Mice Mice, Inbred C57BL Mitochondria, Liver / enzymology* Substrate Specificity Transaminases / isolation & purification, metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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RR-07042/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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106-60-5/Aminolevulinic Acid; EC 2.6.1.-/Transaminases; EC 2.6.1.43/aminolevulinate aminotransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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