Document Detail


Production of a cloned calf using zona-free serial nuclear transfer.
MedLine Citation:
PMID:  15979134     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The efficiency of generating cloned animals following somatic cell nuclear transfer appears to have reached a plateau, despite ongoing research to improve developmental outcomes. A major limitation appears in the restricted nature of the adult/donor cell to de-differentiate to form a totipotent nucleus. Serial nuclear transfer, a modified cloning technique, has increased the developmental competence of amphibian, murine and porcine cloned embryos. This procedure involves a second nuclear transfer step; pronuclear-like cloned nuclei are transferred into pronuclear stage zygotic cytoplasts. The present study reports on the development of a serial nuclear transfer technique in the bovine, based on a zona-free method (hand-made cloning), resulting in the birth of a cloned calf. Comparisons were made between embryos produced by hand-made cloning and serial nuclear transfer. There were no differences between in vitro development or differential cell counts in the blastocysts produced. Transfer of 16 serial hand-made cloned blastocysts resulted in the production of one healthy calf (6%), whereas hand-made cloning resulted in the birth of 1 calf from 23 transferred blastocysts (4%). One serial nuclear transfer pre-term fetus had renal and hepatic abnormalities (previously observed in clones from this cell line). Although it may not be as beneficial in the bovine as in other species, normal placentation (size, placentomes and umbilicus) was encouraging. Refinement of this technique may help to identify species-specific differences in zygotic competence that affect reprogramming of donor cell nuclei and that may improve efficiency.
Authors:
Vanessa J Hall; Nancy T Ruddock; Melissa A Cooney; Natasha A Korfiatis; R Tayfur Tecirlioglu; Shara Downie; Mark Williamson; Andrew J French
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Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2005-06-23
Journal Detail:
Title:  Theriogenology     Volume:  65     ISSN:  0093-691X     ISO Abbreviation:  Theriogenology     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-20     Completed Date:  2006-09-06     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  424-40     Citation Subset:  IM    
Affiliation:
Monash University, Monash Institute of Reproduction and Development, Centre for Early Human Development, 27-31 Wright Street, Clayton, Vic. 3168, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / chemistry
Cattle / embryology,  genetics,  physiology*
Cloning, Organism / methods,  veterinary*
Embryo Transfer / veterinary
Embryo, Mammalian / cytology
Female
Fertilization in Vitro / standards,  veterinary*
Fetal Death / pathology,  veterinary
Karyotyping / veterinary
Nuclear Transfer Techniques*
Oxazines / metabolism
Pregnancy
Zygote / physiology
Chemical
Reg. No./Substance:
0/Oxazines; 0/aceto-orcein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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