Document Detail


Production of chicken chimeras by fusing blastodermal cells with electroporation.
MedLine Citation:
PMID:  11202415     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To establish techniques for producing somatic and germline chimeric chicken by transferring blastodermal cells fused with electroporation. METHODS: Stage-X blastodermal cells isolated from freshly laid fertile unincubated white Leghorn and Rhode Island red chicken eggs were fused with electroporation. The treated cell suspension was transferred to the recovery medium (DMEM containing 10% FBS) and was injected into the subgerminal cavity of recipient unincubated embryos (stage X). RESULTS: Of 177 recipient embryos injected with the fusing blastodermal cells, 6 (3.4%) survived to hatching. Somatic chimerism was examined in the melanocyte of the feather. The presence of feathers originating from the donor cell was observed in 1 bird (16.7%) out of the 6 hatched birds. After 21 days of incubation two birds out of five embryos were subjected to polymerase chain reaction (PCR) analysis for W-chromosome-specific DNA for each tissue. One bird possessed W-chromosome-specific DNA in the stomach, and the other exhibited the same DNA in the left and right gonads and other tissues, but not the stomach. CONCLUSION: Recipient embryo having electrofused blastodermal cells yields somatic and germline chimeric chickens more successfully.
Authors:
S Aritomi; N Fujihara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Asian journal of andrology     Volume:  2     ISSN:  1008-682X     ISO Abbreviation:  Asian J. Androl.     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-02-01     Completed Date:  2001-03-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100942132     Medline TA:  Asian J Androl     Country:  China    
Other Details:
Languages:  eng     Pagination:  271-5     Citation Subset:  IM    
Affiliation:
Division of Animal Resource Science, Faculty of Agriculture, Kyushu University Graduate School, Fukuoka 812-8581, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified / embryology,  genetics,  surgery
Blastoderm / cytology*,  metabolism,  transplantation*
Breeding / methods
Chick Embryo
Chickens / anatomy & histology,  genetics*,  metabolism
Electroporation / methods*
Female
Green Fluorescent Proteins
Luminescent Proteins / genetics
Male
Sex Chromosomes / metabolism
Survival Rate
Testis / embryology
Transplantation Chimera / anatomy & histology,  genetics*
Chemical
Reg. No./Substance:
0/Luminescent Proteins; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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