| Production of chicken chimeras by fusing blastodermal cells with electroporation. | |
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MedLine Citation:
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PMID: 11202415 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To establish techniques for producing somatic and germline chimeric chicken by transferring blastodermal cells fused with electroporation. METHODS: Stage-X blastodermal cells isolated from freshly laid fertile unincubated white Leghorn and Rhode Island red chicken eggs were fused with electroporation. The treated cell suspension was transferred to the recovery medium (DMEM containing 10% FBS) and was injected into the subgerminal cavity of recipient unincubated embryos (stage X). RESULTS: Of 177 recipient embryos injected with the fusing blastodermal cells, 6 (3.4%) survived to hatching. Somatic chimerism was examined in the melanocyte of the feather. The presence of feathers originating from the donor cell was observed in 1 bird (16.7%) out of the 6 hatched birds. After 21 days of incubation two birds out of five embryos were subjected to polymerase chain reaction (PCR) analysis for W-chromosome-specific DNA for each tissue. One bird possessed W-chromosome-specific DNA in the stomach, and the other exhibited the same DNA in the left and right gonads and other tissues, but not the stomach. CONCLUSION: Recipient embryo having electrofused blastodermal cells yields somatic and germline chimeric chickens more successfully. |
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Authors:
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S Aritomi; N Fujihara |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Asian journal of andrology Volume: 2 ISSN: 1008-682X ISO Abbreviation: Asian J. Androl. Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2001-02-01 Completed Date: 2001-03-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100942132 Medline TA: Asian J Androl Country: China |
Other Details:
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Languages: eng Pagination: 271-5 Citation Subset: IM |
Affiliation:
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Division of Animal Resource Science, Faculty of Agriculture, Kyushu University Graduate School, Fukuoka 812-8581, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified / embryology, genetics, surgery Blastoderm / cytology*, metabolism, transplantation* Breeding / methods Chick Embryo Chickens / anatomy & histology, genetics*, metabolism Electroporation / methods* Female Green Fluorescent Proteins Luminescent Proteins / genetics Male Sex Chromosomes / metabolism Survival Rate Testis / embryology Transplantation Chimera / anatomy & histology, genetics* |
| Chemical | |
Reg. No./Substance:
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0/Luminescent Proteins; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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