| Production of chick embryo extract for the cultivation of murine neural crest stem cells. | |
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MedLine Citation:
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PMID: 21178955 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The neural crest arises from the neuro-ectoderm during embryogenesis and persists only temporarily. Early experiments already proofed pluripotent progenitor cells to be an integral part of the neural crest(1). Phenotypically, neural crest stem cells (NCSC) are defined by simultaneously expressing p75 (low-affine nerve growth factor receptor, LNGFR) and SOX10 during their migration from the neural crest(2,3,4,5). These progenitor cells can differentiate into smooth muscle cells, chromaffin cells, neurons and glial cells, as well as melanocytes, cartilage and bone(6,7,8,9). To cultivate NCSC in vitro, a special neural crest stem cell medium (NCSCM) is required(10). The most complex part of the NCSCM is the preparation of chick embryo extract (CEE) representing an essential source of growth factors for the NCSC as well as for other types of neural explants. Other NCSCM ingredients beside CEE are commercially available. Producing CCE using laboratory standard equipment it is of high importance to know about the challenging details as the isolation, maceration, centrifugation, and filtration processes. In this protocol we describe accurate techniques to produce a maximized amount of pure and high quality CEE. |
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Authors:
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Kristian Pajtler; Anna Bohrer; Jochen Maurer; Hubert Schorle; Alexander Schramm; Angelika Eggert; Johannes Hubertus Schulte |
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Publication Detail:
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Type: Journal Article; Video-Audio Media Date: 2010-11-27 |
Journal Detail:
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Title: Journal of visualized experiments : JoVE Volume: - ISSN: 1940-087X ISO Abbreviation: J Vis Exp Publication Date: 2010 |
Date Detail:
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Created Date: 2010-12-23 Completed Date: 2011-01-27 Revised Date: 2013-02-19 |
Medline Journal Info:
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Nlm Unique ID: 101313252 Medline TA: J Vis Exp Country: United States |
Other Details:
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Languages: eng Pagination: - Citation Subset: IM |
Affiliation:
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Department of Pediatric Oncology, University Children's Hospital Essen. kristian.pajtler@uk-essen.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Culture Techniques / methods* Chick Embryo / chemistry* Embryonic Stem Cells / cytology* Mice Neural Crest / cytology* Tissue Extracts / isolation & purification*, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Tissue Extracts |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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