Document Detail


Production and characterization of mouse thymic epithelial cell clones.
MedLine Citation:
PMID:  7512533     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Four thymic epithelial cell lines (TEC) were derived from neonatal CBA and non-obese diabetic (NOD) mouse thymus. From these cell lines a series of clones were produced by limit dilution and these have remained in stable culture for more than 1 year. Morphological characterization indicates that most cells are stellate with numerous short or long processes and ultrastructural studies show both active and quiescent cells with junctional complexes and bundles of tonofibrils. Immunohistochemical and flow cytometric analyses show that the cells express cytokeratin and appear to label for markers characteristic of cortical epithelial cells. Most clones express Thy-1, Pgp-1, ICAM-1, HSA and B220 antigen, but are negative for LFA-1, CD2, Mel 14, Fc receptor, Mac-1, CD4 and CD8. All clones express low to moderate levels of class I MHC but are either negative or extremely low for class II MHC antigen. Most clones secrete IL-6 and granulocyte-macrophage-CSF (GM-CSF) in vitro, but generally do not produce IL-2, IL-3, IL-4 or IFN-gamma.
Authors:
H M Georgiou; D Constantinou
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunology and cell biology     Volume:  72     ISSN:  0818-9641     ISO Abbreviation:  Immunol. Cell Biol.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-05-17     Completed Date:  1994-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8706300     Medline TA:  Immunol Cell Biol     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  57-67     Citation Subset:  IM    
Affiliation:
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Antigens, Surface / metabolism
Biological Markers
Cell Line
Cells, Cultured
Clone Cells
Culture Techniques / methods
Cytokines / biosynthesis
Epithelial Cells
Epithelium / metabolism
Flow Cytometry
Immunoenzyme Techniques
Keratins / metabolism
Mice
Mice, Inbred CBA
Mice, Inbred NOD
Thymus Gland / cytology*,  metabolism
Chemical
Reg. No./Substance:
0/Antigens, Surface; 0/Biological Markers; 0/Cytokines; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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