| Production and characterization of monoclonal and polyclonal antibodies against thyrotropin-releasing hormone. | |
| | |
MedLine Citation:
|
PMID: 7590793 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In this article, two mouse monoclonal antibodies (83-7B5-A1 and 83-6B6-A10) and three rabbit polyclonal antibodies (1118, 8572, and 8577) directed against thyrotropin-releasing hormone (TRH; pGlu-His-Pro-NH2) are described. The anti-TRH antibodies were raised by immunization with a TRH-bovine serum albumin conjugate obtained by coupling of the CO2H group of pGlu-His-Pro-OH to NH2 groups in the protein. The monoclonal antibodies were produced by hybridoma clones obtained by the fusion of SP2/0 myeloma cells with spleen cells of an immunized BALB/c mouse. Both monoclonal antibodies were of the IgG1 (kappa) subclass. Characterization of the anti-TRH antibodies showed that in general they are specific for the pGlu-His moiety. The cross-reactivities for the TRH-like peptides [Glu1]TRH, [Glu2]TRH, and [Phe2]TRH are low, while alterations at the Pro-NH2 moiety of TRH are recognized to varying extents. The specificities of these antibodies are markedly different from those previously obtained using TRH coupled through the histidine residue to protein as the immunogen. |
| | |
Authors:
|
W Klootwijk; L M Vaessen; B F Bernard; J M Rondeel; W J De Greef; T J Visser |
Related Documents
:
|
21524853 - Prevalence of specific igg-antibodies against toxoplasma gondii in domestic turkeys det... 1383483 - Monoclonal antibodies to beta subunit of choriogonadotropin: development and use in a s... 6352103 - Characterization of the humoral immune response in plasmodium falciparum malaria. ii. i... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Hybridoma Volume: 14 ISSN: 0272-457X ISO Abbreviation: Hybridoma Publication Date: 1995 Jun |
Date Detail:
|
Created Date: 1995-12-12 Completed Date: 1995-12-12 Revised Date: 2005-11-17 |
Medline Journal Info:
|
Nlm Unique ID: 8202424 Medline TA: Hybridoma Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 285-90 Citation Subset: IM |
Affiliation:
|
Department of Internal Medicine III, Erasmus University Medical School, Rotterdam, The Netherlands. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Antibodies / chemistry Antibodies, Monoclonal / biosynthesis*, chemistry, isolation & purification Female Mice Mice, Inbred BALB C Molecular Sequence Data Peptide Fragments / immunology Rabbits Thyrotropin-Releasing Hormone / chemistry, immunology* Tyramine / immunology |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies; 0/Antibodies, Monoclonal; 0/Peptide Fragments; 24305-27-9/Thyrotropin-Releasing Hormone; 51-67-2/Tyramine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Production and characterization of monoclonal antibodies directed against the Ah receptor.
Next Document: Theoretical and empirical considerations of resourcefulness.