Document Detail


Production and characterization of monoclonal antibodies specific for Cryptococcus neoformans capsular polysaccharide.
MedLine Citation:
PMID:  2440810     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cryptococcus neoformans is surrounded by a capsular polysaccharide. There are at least four known serotypes of the polysaccharide. The objective of this study was to produce monoclonal antibodies (MAbs) that could be used to study the distribution of epitopes among the serotypes of C. neoformans. BALB/c mice were immunized with cryptococcal polysaccharides of serotype A or D that were coupled to sheep erythrocytes. Splenocytes were isolated, and hybridomas secreting MAbs specific for cryptococcal polysaccharides were isolated. Two hybridomas, designated MAbs 439 and 1255, were produced from mice immunized with serotype A polysaccharide. One hybridoma, designated MAb 302, was produced from mice immunized with serotype D polysaccharide. All three antibodies were of the immunoglobulin G1 isotype. MAb 302 showed a specificity for serotypes A and D in Ouchterlony diffusion, agglutination, and opsonophagocytosis assays. MAb 1255 was reactive with polysaccharides and cells of serotypes A, B, and D. MAb 439 was reactive with polysaccharides and cells of serotypes A, B, C, and D. The reactivity of these MAbs closely matched the distribution of epitopes among cryptococcal polysaccharides predicted in previous studies of polyclonal antibodies reactive with cryptococcal polysaccharides. The ability to produce a MAb against an epitope shared by all four serotypes may have value for the detection of cryptococcal antigens in body fluids.
Authors:
T F Eckert; T R Kozel
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  55     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1987 Aug 
Date Detail:
Created Date:  1987-09-01     Completed Date:  1987-09-01     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1895-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Agglutination
Animals
Antibodies, Monoclonal / diagnostic use,  immunology*
Antigens, Fungal / immunology*
Cryptococcus / immunology*
Cryptococcus neoformans / classification,  immunology*
Epitopes
Immunodiffusion
Macrophages / immunology
Mice
Opsonin Proteins
Polysaccharides / immunology
Serotyping
Grant Support
ID/Acronym/Agency:
AI14209/AI/NIAID NIH HHS; AI24357/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Fungal; 0/Epitopes; 0/Opsonin Proteins; 0/Polysaccharides
Comments/Corrections

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