Document Detail


Production and characterization of monoclonal antibodies against pregnancy-associated plasma protein A.
MedLine Citation:
PMID:  10381824     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pregnancy-associated plasma protein A (PAPP-A) was found to be a good first trimester maternal serum marker, together with free beta-human chorionic gonadotrophin (HCG) subunits, for the biochemical screening of fetal trisomy 21 (Down's syndrome). We have raised monoclonal antibodies (mAbs) against PAPP-A purified from human pregnancy serum. The different antibodies were characterized biochemically by Western blot analysis and in terms of specificity (reaction with non-pregnant and male serum). Their performance in Down's syndrome screening was assessed in comparison with an existing enzyme-linked immunosorbent assay method after labelling of the different mAbs with biotin or horseradish peroxidase. A pair of mAbs was eventually chosen for a double-antibody sandwich protocol. The selected combination was found to have a significantly increased specificity (P = 0.0116) over the method using (purified) polyclonal antibodies, together with slightly increased sensitivity. In our limited number of Down's syndrome pregnancy samples (n = 17) and controls (n = 18), the medians as well as the multiples of the median values (for the affected cases) were comparable between the two methods described.
Authors:
N A Bersinger; A Meisser; T Bessou; P Seguin; M H Birkhäuser; P Bischof
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular human reproduction     Volume:  5     ISSN:  1360-9947     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-08-16     Completed Date:  1999-08-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  675-81     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynaecology, University of Berne, Schanzeneckstrasse 1, Berne CH-3012, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal*
Blotting, Western / methods
Chorionic Gonadotropin, beta Subunit, Human / blood
Down Syndrome / diagnosis*,  embryology*
Electrophoresis, Polyacrylamide Gel / methods
Female
Humans
Male
Pregnancy
Pregnancy Trimester, First / blood*
Pregnancy-Associated Plasma Protein-A / analysis*,  immunology,  metabolism
Prenatal Diagnosis / methods*
Sensitivity and Specificity
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Chorionic Gonadotropin, beta Subunit, Human; EC 3.4.24.-/Pregnancy-Associated Plasma Protein-A

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