Document Detail


Production and characterization of exotoxin(s) of Shigella dysenteriae type 1.
MedLine Citation:
PMID:  1092769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A semicontinuous fermenter system was developed in which broth culture filtrates of Shigella dysenteriae type 1 yielded substantial amounts of exotoxin. Biologic activity of the exotoxin was characterized by means of three assays: the rabbit ileal loop for fluid evocation (enterotoxicity), mouse lethality after parenteral injection (neurotoxicity), and HeLa cell toxicity in vitro (cytotoxicity). Although the culture filtrate was highly active, disc electrophoresis revealed that the toxin is a minor component of the mixture of proteins in the crude preparation, and that the minor representation contrasts with the relative prominence of exotoxins in cultures of other bacteria, such as Vibrio cholerae, Corynebacterium diphtheriae, and Clostridium tetani. Filtrate toxin, when purified by isoelectric focusing in polyacrylamide gel, was found to contain two toxic moieties. One was resolvable as a single band with an isoelectric joint (pI) of 7.25, a molecular weight of 72,000, and all three types of biological activity. The second moiety was isoelectric at pH 6.00, contained two subcomponents, and further contrasted with the pI 7.25 toxin by being more cytotoxic while being devoid of enteroneurotoxic activity.
Authors:
J McIver; G F Grady; G T Keusch
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  131     ISSN:  0022-1899     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  1975 May 
Date Detail:
Created Date:  1975-07-18     Completed Date:  1975-07-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  559-66     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / analysis
Bacteriological Techniques
Biopharmaceutics
Electrophoresis, Disc
Hela Cells / drug effects
Ileum / drug effects
Molecular Weight
Rabbits
Shigella dysenteriae / immunology*
Sodium Dodecyl Sulfate
Spectrophotometry
Toxins, Biological / isolation & purification*,  toxicity
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Toxins, Biological; 151-21-3/Sodium Dodecyl Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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