Document Detail


Production, characterization and applications of a tetanus toxin specific monoclonal antibody T-62.
MedLine Citation:
PMID:  21975055     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Tetanus neurotoxin (TeNT) represents a potent toxin that binds to its receptors on neurons and inhibits the release of neurotransmitters. Additionally, its fragments are used to transport pharmacological substances to neuronal cell bodies. The main objective of this study was the development of a suitable model system to study internalization of the TeNT. We have produced a monoclonal antibody (MoAb) specific for TeNT by hybridoma technology, after immunization of BALB/c mice with tetanus toxoid, and have named it T-62. The immunochemical characteristics of MoAb T-62 were tested using ELISA, PAGE and immunoblotting. Finally, we have used an immunohistochemical method to detect specific binding of MoAb T-62 to TeNT bound to PC 12 cells. Our results show that MoAb T-62 is highly specific for TeNT, even when it is bound to its receptor, and that it could be of considerable importance in studies regarding fundamental research on TeNT receptors, intracellular transport of TeNT, as well as retrograde transport of pharmaceutical substances and non-invasive delivery of polypeptides through the blood brain barrier. In addition, MoAb T-62 is an invaluable tool in TeNT vaccine production as it can be used for the detection of reverse toxicity, which could drastically reduce the need to use animals in these experiments.
Authors:
Vladimir Petrušić; Irena Zivković; Marijana Stojanović; Ivana Stojićević; Emilija Marinković; Ljiljana Dimitrijević
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-3
Journal Detail:
Title:  Acta histochemica     Volume:  -     ISSN:  1618-0372     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370320     Medline TA:  Acta Histochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier GmbH. All rights reserved.
Affiliation:
Institute of Virology, Vaccines and Sera - Torlak, Department of Research and Development, Vojvode Stepe 458, 11221 Belgrade, Serbia.
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