| Production and characterization of an anti-(MUC1 mucin) recombinant diabody. | |
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MedLine Citation:
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PMID: 10235486 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A recombinant diabody fragment based on the anti-MUC1 monoclonal antibody, C595 has been produced in a bacterial expression system. Substitution of a 7-amino-acid linker sequence (Gly6Ser) for the original single-chain (sc)Fv 15-amino-acid linker (Gly4-Ser)3, using polymerase-chain-reaction-based strategies, forces variable heavy (V(H)) and light (V(L)) domains to pair with complementary domains on neighbouring scFv molecules, forming a scFv dimer (diabody). This recombinant protein shows similar binding characteristics to the parental C595 monoclonal antibody. The ability to bind to MUC1 mucin on carcinoma cell surfaces will allow its potential as a diagnostic and therapeutic reagent of clinical utility to be investigated. |
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Authors:
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G Denton; K Brady; B K Lo; A Murray; C R Graves; O D Hughes; S J Tendler; C A Laughton; M R Price |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer immunology, immunotherapy : CII Volume: 48 ISSN: 0340-7004 ISO Abbreviation: Cancer Immunol. Immunother. Publication Date: 1999 Apr |
Date Detail:
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Created Date: 1999-06-01 Completed Date: 1999-06-01 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8605732 Medline TA: Cancer Immunol Immunother Country: GERMANY |
Other Details:
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Languages: eng Pagination: 29-38 Citation Subset: IM |
Affiliation:
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Cancer Research Laboratories, School of Pharmaceutical Sciences, University of Nottingham, University Park, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antibodies, Bispecific / biosynthesis, chemistry, genetics Antibodies, Monoclonal / biosynthesis*, genetics, immunology* Antibody Specificity Dimerization Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin Fragments / biosynthesis*, genetics, immunology* Mice Models, Molecular Molecular Sequence Data Mucin-1 / genetics, immunology* Polymerase Chain Reaction Protein Conformation Recombinant Proteins / biosynthesis*, chemistry, genetics Sequence Homology, Amino Acid Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Bispecific; 0/Antibodies, Monoclonal; 0/Immunoglobulin Fragments; 0/Mucin-1; 0/Recombinant Proteins |
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