Document Detail


Production and characterization of an anti-(MUC1 mucin) recombinant diabody.
MedLine Citation:
PMID:  10235486     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A recombinant diabody fragment based on the anti-MUC1 monoclonal antibody, C595 has been produced in a bacterial expression system. Substitution of a 7-amino-acid linker sequence (Gly6Ser) for the original single-chain (sc)Fv 15-amino-acid linker (Gly4-Ser)3, using polymerase-chain-reaction-based strategies, forces variable heavy (V(H)) and light (V(L)) domains to pair with complementary domains on neighbouring scFv molecules, forming a scFv dimer (diabody). This recombinant protein shows similar binding characteristics to the parental C595 monoclonal antibody. The ability to bind to MUC1 mucin on carcinoma cell surfaces will allow its potential as a diagnostic and therapeutic reagent of clinical utility to be investigated.
Authors:
G Denton; K Brady; B K Lo; A Murray; C R Graves; O D Hughes; S J Tendler; C A Laughton; M R Price
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer immunology, immunotherapy : CII     Volume:  48     ISSN:  0340-7004     ISO Abbreviation:  Cancer Immunol. Immunother.     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-06-01     Completed Date:  1999-06-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8605732     Medline TA:  Cancer Immunol Immunother     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  29-38     Citation Subset:  IM    
Affiliation:
Cancer Research Laboratories, School of Pharmaceutical Sciences, University of Nottingham, University Park, UK.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Antibodies, Bispecific / biosynthesis,  chemistry,  genetics
Antibodies, Monoclonal / biosynthesis*,  genetics,  immunology*
Antibody Specificity
Dimerization
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin Fragments / biosynthesis*,  genetics,  immunology*
Mice
Models, Molecular
Molecular Sequence Data
Mucin-1 / genetics,  immunology*
Polymerase Chain Reaction
Protein Conformation
Recombinant Proteins / biosynthesis*,  chemistry,  genetics
Sequence Homology, Amino Acid
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Antibodies, Bispecific; 0/Antibodies, Monoclonal; 0/Immunoglobulin Fragments; 0/Mucin-1; 0/Recombinant Proteins

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