| Production of carboxylic acids from hydrolyzed corn meal by immobilized cell fermentation in a fibrous-bed bioreactor. | |
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MedLine Citation:
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PMID: 11848378 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Corn meal hydrolyzed with amylases was used as the carbon source for producing acetic, propionic, and butyric acids via anaerobic fermentations. In this study, corn meal, containing 75% (w/w) starch, 20% (w/w) fibers, and 1.5% (w/w) protein, was first hydrolyzed using amylases at 60 degrees C. The hydrolysis yielded approximately 100% recovery of starch converted to glucose and 17.9% recovery of protein. The resulting corn meal hydrolyzate was then used, after sterilization, for fermentation studies. A co-culture of Lactococcus lactis and Clostridium formicoaceticum was used to produce acetic acid from glucose. Propionibacterium acidipropionici was used for propionic acid fermentation, and Clostridium tyrobutylicum was used for butyric acid production. These cells were immobilized on a spirally wound fibrous matrix packed in a fibrous-bed bioreactor (FBB) developed for multi-phase biological reactions or fermentation. The bioreactor was connected to a stirred-tank fermentor that provided pH and temperature controls via medium circulation. The fermentation system was operated at the recycle batch mode. Temperature and pH were controlled at 37 degrees C and 7.6, respectively, for acetic acid fermentation, 32 degrees C and 6.0, respectively, for propionic acid fermentation, and 37 degrees C and 6.0, respectively, for butyric acid production. The fermentation demonstrated a yield of approximately 100% and a volumetric productivity of approximately 1 g/(1 h) for acetic acid production. The propionic acid fermentation achieved an approximately 60% yield and a productivity of 2.12 g/(1 h), whereas the butyric acid fermentation obtained an approximately 50% yield and a productivity of 6.78 g/(1 h). These results were comparable to, or better than those fermentations using chemically defined media containing glucose as the substrate, suggesting that these carboxylic acids can be efficiently produced from direct fermentation of corn meal hydrolyzate. The corn fiber present as suspended solids in the corn meal hydrolyzate did not cause operating problem to the immobilized cell bioreactor as is usually encountered by conventional immobilized cell bioreactor systems. It is concluded that the FBB technology is suitable for producing value-added biochemicals directly from agricultural residues or commodities such as corn meal. |
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Authors:
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Yu Liang Huang; Zetang Wu; Likun Zhang; Chun Ming Cheung; Shang-Tian Yang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bioresource technology Volume: 82 ISSN: 0960-8524 ISO Abbreviation: Bioresour. Technol. Publication Date: 2002 Mar |
Date Detail:
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Created Date: 2002-02-18 Completed Date: 2002-08-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9889523 Medline TA: Bioresour Technol Country: England |
Other Details:
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Languages: eng Pagination: 51-9 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, The Ohio State University, Columbus 43210, USA. ylhuang@k-online.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetic Acid
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metabolism* Amylases / metabolism Bioreactors / microbiology Butyric Acid / metabolism* Cells, Immobilized / metabolism Clostridium / metabolism Fermentation / physiology Hydrolysis Lactococcus lactis / metabolism Propionibacterium / metabolism Propionic Acids / metabolism* Zea mays / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Propionic Acids; 107-92-6/Butyric Acid; 64-19-7/Acetic Acid; 79-09-4/propionic acid; EC 3.2.1.-/Amylases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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