Document Detail


Production of biomass and extracellular 5-aminolevulinic acid by Rhodopseudomonas palustris KG31 under light and dark conditions using volatile fatty acid.
MedLine Citation:
PMID:  21353636     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Kinetic parameters for growth and extracellular 5-aminolevulinic acid (ALA) production of Rhodopseudomonas palustris KG31 under light and dark conditions in a medium containing volatile fatty acids (VFA) as the carbon sources were estimated using a Gompertz model. The lag phase for growth and the maximum specific growth rate under microaerobic-light cultivations were 7.29-12.49h and 0.038-0.094 h(-1), respectively, whereas under aerobic-dark cultivations, they were 2.03-14.25h and 0.016-0.022 h(-1), respectively. The lag phase for extracellular ALA production and the maximum specific extracellular ALA production rate under microaerobic-light cultivations (15.72-24.74h and 0.222-0.299 h(-1), respectively) were better than those obtained under aerobic-dark cultivations (24.57-44.84h and 0.103-0.215 h(-1), respectively). The biomass and the extracellular ALA yields of 39.66-56.25 gDCW/l/molC, and 148.47-245.75μM/molC, respectively, under microaerobic-light cultivations were higher than of those obtained under aerobic-dark conditions. An enhancement of extracellular ALA production under aerobic-dark conditions revealed that the ALA yield was markedly increased 8-fold (48.36μM) by the addition of 10mM succinate, 4.5mM glycine, and 15mM levulinic acid (LA). By controlling dissolved oxygen (DO) and pH values, a maximum extracellular ALA yield of 66.38μM was found. The degradation rate of ALA in the culture broth was closely related to the pH value.
Authors:
Wanna Choorit; Angkana Saikeur; Pichit Chodok; Poonsuk Prasertsan; Duangporn Kantachote
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-23
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  -     ISSN:  1347-4421     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
Affiliation:
School of Agricultural Technology, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
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