Document Detail


Production of biofilm and quorum sensing by Escherichia coli O157:H7 and its transfer from contact surfaces to meat, poultry, ready-to-eat deli, and produce products.
MedLine Citation:
PMID:  19465248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Multistate outbreaks of Escherichia coli O157:H7 infections through consumption of contaminated foods including produce products have brought a great safety concern. The objectives of this study were to determine the effect of biofilm and quorum sensing production on the attachment of E. coli O157:H7 on food contact surfaces and to evaluate the transfer of the pathogen from the food contact to various food products. E. coli O157:H7 produced maximum levels of AI-2 signals in 12 h of incubation in tested meat, poultry, and produce broths and subsequently formed strong biofilm in 24 h of incubation. In general, E. coli O157:H7 formed stronger biofilm on stainless steel than glass. Furthermore, E. coli O157:H7 that had attached on the surface of stainless steel was able to transfer to meat, poultry, ready-to-eat deli, and produce products. Strong attachment of the transferred pathogen on produce products (cantaloupe, lettuce, carrot, and spinach) was detected (>10(3) CFU/cm2) even after washing these products with water. Our findings suggest that biofilm formation by E. coli O157:H7 on food contact surfaces can be a concern for efficient control of the pathogen particularly in produce products that require no heating or cooking prior to consumption.
Authors:
Karen Silagyi; Shin-Hee Kim; Y Martin Lo; Cheng-i Wei
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-18
Journal Detail:
Title:  Food microbiology     Volume:  26     ISSN:  1095-9998     ISO Abbreviation:  Food Microbiol.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-05-25     Completed Date:  2009-07-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8601127     Medline TA:  Food Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  514-9     Citation Subset:  IM    
Affiliation:
Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion / physiology*
Biofilms / growth & development*
Colony Count, Microbial
Consumer Product Safety
Environmental Microbiology
Escherichia coli O157 / physiology*
Food Contamination / analysis*,  prevention & control
Fruit / microbiology
Glass
Meat / microbiology
Meat Products / microbiology
Quorum Sensing*
Stainless Steel
Time Factors
Vegetables / microbiology
Chemical
Reg. No./Substance:
12597-68-1/Stainless Steel

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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