Document Detail

Production of bioengineered cancer tissue constructs in vitro: epithelium-mesenchyme heterotypic interactions.
MedLine Citation:
PMID:  11573818     Owner:  NLM     Status:  MEDLINE    
A few models have been established to study cancer cells in vitro. However, the cellular interactions have rarely been studied specifically using bioengineered cancer constructs combining human carcinoma cells and tumor-associated fibroblasts. We developed an in vitro model of tridimensional bioengineered cancer tissue constructs (bCTC) by seeding mammary epithelial cancer cells or normal keratinocytes over a mesenchymal layer containing tumor-derived fibroblastic cells or normal skin fibroblasts. After the introduction of epithelial cells, each construct was cultured for another 10 d. Histologic analyses showed that carcinoma cell lines could invade the subjacent mesenchymal layer and that the capacity to migrate was related to the invasive potential of cancer cells and the type of fibroblasts used, while noninvasive populations did not. Of the tested epithelial cells, MDA-MB-231 and, to a lesser degree, HDQ-P1 cell lines were invasive, and the invasion was deeper into the mesenchymal component containing tumor-derived fibroblasts. However, with normal skin fibroblasts, the mesenchymal layer was degraded twice faster than with tumor-derived fibroblastic cells. MDA-MB-231 cells and normal keratinocytes induced the highest level of gelatinase B, and the level was lowest with the MCF-7 cell line. The activated form of gelatinase B was, however, induced to the highest levels in the keratinocyte-seeded bCTC containing tumor-derived but not normal fibroblasts. MDA-MB-231 was the only epithelial cancer cell line whose activity of gelatinase A was reduced when cocultured with tumor-derived fibroblasts but not under normal fibroblast stimulation. Finally, a 50/48-kDa gelatinase band has been observed in bCTCs with noninvasive epithelial cells only. Our study demonstrates the selective secretion of gelatinases according to the phenotype of the cells seeded in the various bCTCs.
C S Wang; F Goulet; F Auger; N Tremblay; L Germain; B Têtu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  37     ISSN:  1071-2690     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:    2001 Jul-Aug
Date Detail:
Created Date:  2001-09-27     Completed Date:  2002-02-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  United States    
Other Details:
Languages:  eng     Pagination:  434-9     Citation Subset:  IM    
Centre de Recherche en Cancérologie, Centre Hospitalier Universitaire de Québec, L'Hôtel-Dieu de Québec, Canada.
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MeSH Terms
Breast Neoplasms / pathology
Cell Communication*
Coculture Techniques
Culture Media
Epithelial Cells / pathology*
Keratins / analysis
Matrix Metalloproteinase 2 / analysis
Matrix Metalloproteinase 9 / analysis
Matrix Metalloproteinases / metabolism
Mesoderm / pathology*
Neoplasm Invasiveness
Neoplasms / pathology*
Tumor Cells, Cultured
Vimentin / analysis
Reg. No./Substance:
0/Culture Media; 0/Vimentin; 68238-35-7/Keratins; EC 3.4.24.-/Matrix Metalloproteinases; EC Metalloproteinase 2; EC Metalloproteinase 9

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