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PRODUCTION OF BCG VACCINE IN A LIQUID MEDIUM CONTAINING TWEEN 80 AND A SOLUBLE FRACTION OF HEATED HUMAN SERUM : II. ANTIGENICITY OF THE CULTURE AFTER VARIOUS PERIODS OF STORAGE.
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MedLine Citation:
PMID:  19871704     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Groups of guinea pigs were vaccinated by the intracutaneous route with cultures of BCG grown in a liquid medium containing Tween 80 and the soluble fraction of heated human serum. After the cultures had been stored at 4 degrees C. for various periods of time, the antigenic response was compared with that of another group of guinea pigs receiving standard BCG vaccine prepared by the conventional technique. The local lesions occurring at the site of injection of cultures in Tween-serum filtrate medium were more severe than those produced by the standard vaccine. It was shown that this difference was probably due to the much larger number of viable bacilli in the former preparations. A marked degree of sensitization could be produced with culture dilutions containing as few as 10 viable units (single bacilli or small clumps). Slightly larger doses of BCG led to the highest degree of tuberculin allergy detectable by the technique employed. Further increases in the dose of vaccine failed to alter the level of sensibility when the animals were tested with tuberculin 5 weeks after vaccination. The same degree of sensitization was achieved by vaccination with 0.1 cc. of either the standard vaccine or any of the fresh or stored cultures in Tween-serum filtrate medium. It was shown that these doses contained numbers of living bacilli far greater than the minimal number required to induce maximal sensitization. Under the conditions used, the guinea pigs vaccinated with cultures of BCG (fresh or stored) grown in the Tween-serum filtrate medium exhibited a marked degree of resistance to subcutaneous infection with virulent tubercle bacilli.
Authors:
F Fenner; R J Dubos
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of experimental medicine     Volume:  91     ISSN:  0022-1007     ISO Abbreviation:  J. Exp. Med.     Publication Date:  1950 Feb 
Date Detail:
Created Date:  2010-06-23     Completed Date:  2010-06-23     Revised Date:  2010-09-28    
Medline Journal Info:
Nlm Unique ID:  2985109R     Medline TA:  J Exp Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  269-84     Citation Subset:  -    
Affiliation:
Laboratories of The Rockefeller Institute for Medical Research.
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Full Text
Journal Information
Journal ID (nlm-ta): J Exp Med
ISSN: 0022-1007
ISSN: 1540-9538
Publisher: The Rockefeller University Press
Article Information
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Copyright © Copyright, 1950, by The Rockefeller Institute for Medical Research New York
Received Day: 16 Month: 11 Year: 1949
Print publication date: Day: 28 Month: 2 Year: 1950
Volume: 91 Issue: 3
First Page: 269 Last Page: 284
ID: 2135961
PubMed Id: 19871704

PRODUCTION OF BCG VACCINE IN A LIQUID MEDIUM CONTAINING TWEEN 80 AND A SOLUBLE FRACTION OF HEATED HUMAN SERUM : II. ANTIGENICITY OF THE CULTURE AFTER VARIOUS PERIODS OF STORAGE
Frank Fenner
René J. Dubos
From the Laboratories of The Rockefeller Institute for Medical Research


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