Document Detail


Production of bacterial endoglucanase from pretreated oil palm empty fruit bunch by bacillus pumilus EB3.
MedLine Citation:
PMID:  18929997     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, endoglucanase was produced from oil palm empty fruit bunch (OPEFB) by a locally isolated aerobic bacterium, Bacillus pumilus EB3. The effects of the fermentation parameters such as initial pH, temperature, and nitrogen source on the endoglucanase production were studied using carboxymethyl cellulose (CMC) as the carbon source. Endoglucanase from B. pumilus EB3 was maximally secreted at 37 degrees C, initial pH 7.0 with 10 g/l of CMC as carbon source, and 2 g/l of yeast extract as organic nitrogen source. The activity recorded during the fermentation was 0.076 U/ml. The productivity of the enzyme increased twofold when 2 g/l of yeast extract was used as the organic nitrogen supplement as compared to the non-supplemented medium. An interesting finding from this study is that pretreated OPEFB medium showed comparable results to CMC medium in terms of enzyme production with an activity of 0.063 U/ml. As OPEFB is an abundant solid waste at palm oil mills, it has the potential of acting as a substrate in cellulase production.
Authors:
Hidayah Ariffin; Mohd Ali Hassan; Umi Kalsom Md Shah; Norhafizah Abdullah; Farinazleen Mohd Ghazali; Yoshihito Shirai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  106     ISSN:  1347-4421     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-10-20     Completed Date:  2009-01-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  231-6     Citation Subset:  IM    
Affiliation:
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
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MeSH Terms
Descriptor/Qualifier:
Arecaceae / microbiology*
Bacillus / enzymology*
Bioreactors / microbiology*
Cell Culture Techniques / methods*
Fruit / microbiology*
Plant Oils / chemistry*,  metabolism*
Ribonucleases / metabolism*
Chemical
Reg. No./Substance:
0/Plant Oils; 8002-75-3/palm oil; EC 3.1.-/Ribonucleases; EC 3.1.4.-/Bacillus pumilus ribonuclease

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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