Document Detail

Production of aromatics in Saccharomyces cerevisiae-A feasibility study.
MedLine Citation:
PMID:  22579724     Owner:  NLM     Status:  Publisher    
Aromatics are amongst the most important bulk feedstocks for the chemical industry, however, no viable bioprocess exists today and production is still dependent on petro-chemistry. In this article the production of aromatic precursors such as p-hydroxybenzoic acid (PHBA) and p-amino benzoic acid (PABA) in Saccharomyces cerevisiae was evaluated using metabolic network analysis. Theoretical mass yields for PHBA and for PABA obtained by metabolic network analysis were 0.58 and 0.53gg(glucose)(-1), respectively. A major setback for microbial production of aromatics is the high toxicity of the products. Therefore, PHBA and PABA toxicity was evaluated in S. cerevisiae. Minimal inhibitory concentrations of 38.3gL(-1) for PHBA and 0.62gL(-1) for PABA were observed. However, PABA toxicity could be alleviated in adaptation experiments. Finally, metabolic engineering was used to create proof of principle first generation strains of S. cerevisiae. Overall accumulation of 650μM PHBA and 250μM PABA could be achieved.
Jens O Krömer; Dariela Nunez-Bernal; Nils Averesch; Jennifer Hampe; Javier Varela; Cristian Varela
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-3
Journal Detail:
Title:  Journal of biotechnology     Volume:  -     ISSN:  1873-4863     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, Queensland, Australia; Centre for Microbial Electrosynthesis (CEMES), Advanced Water Management Centre, Gehrmann labs (Bldg 60-620), The University of Queensland, Queensland 4072, Australia.
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