Document Detail

Production of aromatic compounds by metabolically engineered Escherichia coli with an expanded shikimate pathway.
MedLine Citation:
PMID:  22752168     Owner:  NLM     Status:  MEDLINE    
Escherichia coli was metabolically engineered by expanding the shikimate pathway to generate strains capable of producing six kinds of aromatic compounds, phenyllactic acid, 4-hydroxyphenyllactic acid, phenylacetic acid, 4-hydroxyphenylacetic acid, 2-phenylethanol, and 2-(4-hydroxyphenyl)ethanol, which are used in several fields of industries including pharmaceutical, agrochemical, antibiotic, flavor industries, etc. To generate strains that produce phenyllactic acid and 4-hydroxyphenyllactic acid, the lactate dehydrogenase gene (ldhA) from Cupriavidus necator was introduced into the chromosomes of phenylalanine and tyrosine overproducers, respectively. Both the phenylpyruvate decarboxylase gene (ipdC) from Azospirillum brasilense and the phenylacetaldehyde dehydrogenase gene (feaB) from E. coli were introduced into the chromosomes of phenylalanine and tyrosine overproducers to generate phenylacetic acid and 4-hydroxyphenylacetic acid producers, respectively, whereas ipdC and the alcohol dehydrogenase gene (adhC) from Lactobacillus brevis were introduced to generate 2-phenylethanol and 2-(4-hydroxyphenyl)ethanol producers, respectively. Expression of the respective introduced genes was controlled by the T7 promoter. While generating the 2-phenylethanol and 2-(4-hydroxyphenyl)ethanol producers, we found that produced phenylacetaldehyde and 4-hydroxyphenylacetaldehyde were automatically reduced to 2-phenylethanol and 2-(4-hydroxyphenyl)ethanol by endogenous aldehyde reductases in E. coli encoded by the yqhD, yjgB, and yahK genes. Cointroduction and cooverexpression of each gene with ipdC in the phenylalanine and tyrosine overproducers enhanced the production of 2-phenylethanol and 2-(4-hydroxyphenyl)ethanol from glucose. Introduction of the yahK gene yielded the most efficient production of both aromatic alcohols. During the production of 2-phenylethanol, 2-(4-hydroxyphenyl)ethanol, phenylacetic acid, and 4-hydroxyphenylacetic acid, accumulation of some by-products were observed. Deletion of feaB, pheA, and/or tyrA genes from the chromosomes of the constructed strains resulted in increased desired aromatic compounds with decreased by-products. Finally, each of the six constructed strains was able to successfully produce a different aromatic compound as a major product. We show here that six aromatic compounds are able to be produced from renewable resources without supplementing with expensive precursors.
Daisuke Koma; Hayato Yamanaka; Kunihiko Moriyoshi; Takashi Ohmoto; Kiyofumi Sakai
Related Documents :
21734438 - Comparative analysis of skin surface lipids of the labia majora, inner thigh, and forearm.
4342208 - The fractionation of phosphatidylinositol into molecular species by thin-layer chromato...
3957918 - Characterization of a diphosphonopentaosylceramide containing 3-o-methylgalactose from ...
20499938 - Nanotube formation through the continuous one-dimensional fusion of hollow nanocapsules...
1970138 - Quinolinate neurotoxicity and glutamatergic structures.
22312728 - Ferric reducing, antiradical and beta-carotene bleaching activities of nicotinic acid a...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-06-29
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-10     Completed Date:  2012-12-11     Revised Date:  2014-01-24    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6203-16     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Alcohol Oxidoreductases / genetics*,  metabolism*
Azospirillum brasilense / enzymology,  genetics
Benzene Derivatives / metabolism*
Cupriavidus necator / enzymology,  genetics
Escherichia coli / genetics*,  metabolism*
Escherichia coli Proteins / genetics*,  metabolism*
Gene Expression
Lactobacillus brevis / enzymology,  genetics
Metabolic Engineering*
Podoviridae / genetics
Promoter Regions, Genetic
Shikimic Acid / metabolism*
Reg. No./Substance:
0/Benzene Derivatives; 0/Escherichia coli Proteins; 29MS2WI2NU/Shikimic Acid; EC 1.1.-/Alcohol Oxidoreductases; EC 1.1.-/YjgB protein, E coli

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Methane-cycling microbial communities and methane emission in natural and restored peatlands.
Next Document:  Protection of Bacillus pumilus spores by catalases.