| Production of arachidonic acid metabolites by the colon adenocarcinoma cell line HT29 cl.19A and their effect on chloride secretion. | |
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MedLine Citation:
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PMID: 9215866 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Eicosanoids were found in large amounts in the colonic mucosa of patients suffering from inflammatory bowel diseases and colonic adenocarcinoma. The aim of this study was to evaluate the role of the intestinal epithelial cells in the arachidonic acid metabolism and their functional response to certain eicosanoids. We used the human adenocarcinoma epithelial cell line HT29 cl.19A cell, which is an in vitro model of colon carcinoma and ion transport. These cells were found to express 5- and 15-lipoxygenase, leukotriene A4 hydrolase and cyclooxygenase-1 and -2 mRNAs. We observed an arachidonic acid metabolism via 5-lipoxygenase pathway despite the lack of FLAP mRNA expression and that certain eicosanoids such as hydroperoxy- and hydroxyeicosatetraenoic acids stimulate chloride secretion. |
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Authors:
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S Battu; G Clement; M Heyman; J M Wal; J Cook-Moreau; J F Desjeux; J L Beneytout |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cancer letters Volume: 116 ISSN: 0304-3835 ISO Abbreviation: Cancer Lett. Publication Date: 1997 Jun |
Date Detail:
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Created Date: 1997-07-28 Completed Date: 1997-07-28 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: IRELAND |
Other Details:
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Languages: eng Pagination: 213-23 Citation Subset: IM |
Affiliation:
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ERS-CNRS No. 6101, Facultés de Médecine et de Pharmacie, Limoges, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arachidonic Acid
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metabolism* Chlorides / metabolism* HT29 Cells / metabolism* Humans Hydroxyeicosatetraenoic Acids / metabolism Leukotriene B4 / metabolism Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Chlorides; 0/Hydroxyeicosatetraenoic Acids; 506-32-1/Arachidonic Acid; 71160-24-2/Leukotriene B4; 73945-47-8/15-hydroxy-5,8,11,13-eicosatetraenoic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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