| Production of the apoptotic cellular mediator 4-Methylthio-2-oxobutyric acid by using an enzymatic stirred tank reactor with in situ product removal. | |
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MedLine Citation:
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PMID: 18092800 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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d-Amino acid oxidase (DAAO) was used to study the oxidative deamination of racemic mixtures of d,l-methionine in its soluble and immobilized forms and thus obtain the corresponding alpha-keto acid. The soluble enzyme form was obtained from a Trigonopsis variabilis CBS 4095 extract, free of l-amino acid oxidase, and was co-immobilized with a 200-fold excess of catalase to avoid the undesirable side reaction of H2O2 with the alpha-keto acid, which would otherwise render its corresponding decarboxylated acid, the 3-methylthiopropionic acid (MTPA). With this biocatalyst, quantitative conversion (>98%) of d-methionine into the alpha-keto acid 4-methylthio-2-oxobutyric acid (MTOB) and into MTPA was achieved using 5 mg.mL(-1) of biocatalyst at pH 8.0, 25 degrees C, and pure oxygen at 3 vvm. A stirred tank reactor with in situ product removal (STR-ISPR) was developed to avoid conversion of MTOB into MTPA. The reaction medium was re-circulated through a strong anion exchange column (Amberlite IRA-400). This resulted in the complete removal of MTOB from the reaction medium. After the reaction, the reaction products were eluted sequentially with water (l-methionine), 10 mM HCl (MTPA), and 0.5 M HCl (MTOB). After elution, MTOB was crystallized to its sodium salt. |
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Authors:
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Miguel García-García; Irene Martínez-Martínez; Alvaro Sánchez-Ferrer; Francisco García-Carmona |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-20 |
Journal Detail:
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Title: Biotechnology progress Volume: 24 ISSN: 8756-7938 ISO Abbreviation: Biotechnol. Prog. Publication Date: 2008 Jan-Feb |
Date Detail:
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Created Date: 2008-02-01 Completed Date: 2008-06-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8506292 Medline TA: Biotechnol Prog Country: United States |
Other Details:
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Languages: eng Pagination: 187-91 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology-A, Faculty of Biology, University of Murcia, Campus Espinardo, E-30071 Murcia, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Ascomycota
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enzymology Bioreactors* D-Amino-Acid Oxidase / metabolism* Magnetic Resonance Spectroscopy Methionine / analogs & derivatives*, chemistry, metabolism Molecular Structure |
| Chemical | |
Reg. No./Substance:
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583-92-6/2-keto-4-methylthiobutyric acid; 63-68-3/Methionine; EC 1.4.3.3/D-Amino-Acid Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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