| Production of antiserum to CRF associated with adrenal atrophy in a rabbit. | |
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MedLine Citation:
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PMID: 6300809 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Corticotropin releasing factor (CRF) was recently isolated from ovine hypothalami by its ability to stimulate adrenocorticotropin (ACTH) and beta-endorphin release from dispersed rat pituitary cells. Intramuscular injection of synthetic ovine CRF conjugated to bovine thyroglobulin with 1-ethyl-3(3-dimethylaminopropyl) carbodiimide and emulsified with Freund's complete adjuvant into a random bred New Zealand white rabbit resulted in antiserum production to CRF associated with adrenal atrophy. A decrease in the level of plasma corticosteroids was associated with an increase in mean total binding of 125I-N-Tyr-CRF. Upon sacrifice, a decrease in pituitary content of ACTH and a decrease in adrenal weight and content of corticosteroids was observed in the rabbit producing antiserum to CRF. Adrenal atrophy was histologically verified with an observed decrease in the adrenal cortical zone not reflected in the zona glomerulosa. Individual cells were relatively larger either with more abundant pale cytoplasm or with distinctly vacuolated cytoplasm. The results presented here are consistent with a physiologically necessary role for this CRF or peptides with similar structures in the hypothalamic-pituitary-adrenal axis. |
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Authors:
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R L Moldow; A J Fischman |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Peptides Volume: 3 ISSN: 0196-9781 ISO Abbreviation: Peptides Publication Date: 1982 Nov-Dec |
Date Detail:
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Created Date: 1983-05-27 Completed Date: 1983-05-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8008690 Medline TA: Peptides Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 989-93 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Cortex Hormones
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blood Adrenal Glands / metabolism, pathology* Adrenocorticotropic Hormone / metabolism Animals Antibody Formation Atrophy / metabolism Corticotropin-Releasing Hormone / immunology*, physiology Immune Sera / pharmacology* Pituitary Gland / metabolism Rabbits Radioimmunoassay Sheep |
| Chemical | |
Reg. No./Substance:
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0/Adrenal Cortex Hormones; 0/Immune Sera; 9002-60-2/Adrenocorticotropic Hormone; 9015-71-8/Corticotropin-Releasing Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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