| Production of alpha, omega-alkanediols using Escherichia coli expressing a cytochrome P450 from Acinetobacter sp. OC4. | |
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MedLine Citation:
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PMID: 16794317 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Our biotransformation using Escherichia coli expressing a cytochrome P450 (CYP) belonging to the CYP153A family from Acinetobacter sp. OC4 produced a great amount of 1-octanol (2,250 mg per liter) from n-octane after 24 h of incubation. This level of production is equivalent to the maximum level previously achieved in biotransformation experiments of alkanes. In addition, the initial production rate of 1-octanol was maintained throughout the entire incubation period. These results indicate that we have achieved the functional and stable expression of a CYP in E. coli for the first time. Further, our biotransformation system showed alpha,omega-diterminal oxidation activity of n-alkanes, and a large amount of 1,8-octanediol (722 mg per liter) was produced from 1-octanol after 24 h of incubation. This is the first report on the bioproduction of alpha,omega-alkanediols from n-alkanes or 1-alkanols. |
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Authors:
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Tadashi Fujii; Tatsuya Narikawa; Futoshi Sumisa; Akira Arisawa; Koji Takeda; Junichi Kato |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 70 ISSN: 0916-8451 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-23 Completed Date: 2006-08-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 1379-85 Citation Subset: IM |
Affiliation:
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Bioresource Laboratories, Mercian Corporation, Shizuoka. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acinetobacter
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classification*,
enzymology*,
genetics Alkanes / chemistry*, metabolism* Cell Extracts Cytochrome P-450 Enzyme System / genetics, isolation & purification, metabolism* Enzyme Stability Escherichia coli / genetics, metabolism* Gene Expression Gene Expression Regulation, Enzymologic Isomerism Mixed Function Oxygenases / genetics, isolation & purification, metabolism* Octanes / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Alkanes; 0/Cell Extracts; 0/Octanes; 111-65-9/octane; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Mixed Function Oxygenases; EC 1.14.-/cytochrome P-450 CYP153 (Acinetobacter) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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