Document Detail


Production of alpha, omega-alkanediols using Escherichia coli expressing a cytochrome P450 from Acinetobacter sp. OC4.
MedLine Citation:
PMID:  16794317     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our biotransformation using Escherichia coli expressing a cytochrome P450 (CYP) belonging to the CYP153A family from Acinetobacter sp. OC4 produced a great amount of 1-octanol (2,250 mg per liter) from n-octane after 24 h of incubation. This level of production is equivalent to the maximum level previously achieved in biotransformation experiments of alkanes. In addition, the initial production rate of 1-octanol was maintained throughout the entire incubation period. These results indicate that we have achieved the functional and stable expression of a CYP in E. coli for the first time. Further, our biotransformation system showed alpha,omega-diterminal oxidation activity of n-alkanes, and a large amount of 1,8-octanediol (722 mg per liter) was produced from 1-octanol after 24 h of incubation. This is the first report on the bioproduction of alpha,omega-alkanediols from n-alkanes or 1-alkanols.
Authors:
Tadashi Fujii; Tatsuya Narikawa; Futoshi Sumisa; Akira Arisawa; Koji Takeda; Junichi Kato
Related Documents :
23279227 - Multidrug-resistant bacteria among patients treated in foreign hospitals: management co...
24482577 - Implementation of innovative pulsed xenon ultraviolet (px-uv) environmental cleaning in...
23326047 - Spectrum of acute kidney injury in the himalayan region.
24214797 - The impact of facecards on patients' knowledge, satisfaction, trust, and agreement with...
1342917 - Pollen calendar of seville and its relation to allergies.
12836907 - Antecedents of hospital ownership conversions, mergers, and closures.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  70     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-23     Completed Date:  2006-08-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1379-85     Citation Subset:  IM    
Affiliation:
Bioresource Laboratories, Mercian Corporation, Shizuoka.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acinetobacter / classification*,  enzymology*,  genetics
Alkanes / chemistry*,  metabolism*
Cell Extracts
Cytochrome P-450 Enzyme System / genetics,  isolation & purification,  metabolism*
Enzyme Stability
Escherichia coli / genetics,  metabolism*
Gene Expression
Gene Expression Regulation, Enzymologic
Isomerism
Mixed Function Oxygenases / genetics,  isolation & purification,  metabolism*
Octanes / metabolism
Chemical
Reg. No./Substance:
0/Alkanes; 0/Cell Extracts; 0/Octanes; 111-65-9/octane; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Mixed Function Oxygenases; EC 1.14.-/cytochrome P-450 CYP153 (Acinetobacter)

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Efficient production of 2-deoxyribose 5-phosphate from glucose and acetaldehyde by coupling of the a...
Next Document:  Bioavailability of glucosyl hesperidin in rats.