| Production of synthetic methionine-free and synthetic methionine-limited alpha casein: protein foodstuff for patients with homocystinuria due to cystathionine beta-synthase deficiency. | |
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MedLine Citation:
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PMID: 20033268 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to evaluate the possibility that synthetic forms of methionine-free alpha-casein and methionine-limited alpha casein could be produced by recombinant means to form the basis for developing an industrial-scale process for the provision of a foodstuff suitable for patients with homocystinuria due to cystathionine beta-synthase (CBS) deficiency. As a first step, two forms of alpha casein gene, encoding methionine-free alpha casein (Fcas) or a methionine-limited alpha casein (Mcas), were synthesised and expressed in Escherichia coli. Using the overexpression vector pET28a, both genes were highly expressed in E. coli in soluble form as well as in inclusion bodies. The two recombinant proteins were purified by the one step methods using the fused His-tag and the Ni(2+)column and validated by Western blot analysis. This work paves the way for industrial-scale production of proteins suitable for patients with homocystinuria due to CBS deficiency. |
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Authors:
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Sayed K Goda; Yasmin W Abu Aqel; Mai R Al-Aswad; Fatma A B Rashedy; Amr S Mohamed |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The protein journal Volume: 29 ISSN: 1875-8355 ISO Abbreviation: Protein J. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-20 Completed Date: 2010-03-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101212092 Medline TA: Protein J Country: Netherlands |
Other Details:
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Languages: eng Pagination: 44-9 Citation Subset: IM |
Affiliation:
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College of Arts and Sciences, Qatar University, P.O. Box 2713, Doha, Qatar. sayed.goda@qu.edu.qa |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Caseins
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analysis,
genetics,
isolation & purification,
metabolism* Cloning, Molecular Cystathionine beta-Synthase / deficiency* Escherichia coli / genetics, metabolism Homocystinuria / diet therapy, enzymology* Humans Methionine / analysis*, genetics, metabolism Protein Engineering* Recombinant Proteins / analysis, genetics, isolation & purification, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Caseins; 0/Recombinant Proteins; 63-68-3/Methionine; EC 4.2.1.22/Cystathionine beta-Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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