Document Detail


Production of Sm37-GAPDH, a major therapeutical target in human schistosomiasis.
MedLine Citation:
PMID:  10712729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme in the glycolytic metabolism and the production of energy. This probably explains why GAPDH was evidenced as a major therapeutical target in several parasitic diseases; either as a vaccine candidate or as a target for chemotherapeutic treatments. Schistosoma mansoni GAPDH (Sm37-GAPDH) is one of the main schistosome vaccine candidates. The production of recombinant Sm37-GAPDH is essential to evaluate the ability of this molecule to induce protective immunity in animals and possibly in humans. The cDNA encoding Sm37-GAPDH has been cloned and sequenced. In addition, five B cell (including the major B-cell epitope Sm35-5) and two T cell epitopes have been localized on the molecule. Different expression systems have been evaluated in respect with the production yield and the GAPDH enzymatic activity. Some of them have led to either a high production of insoluble material (E. coli) or to an inactive enzyme (Pischia pastoris). The present article describes the production setting of rSm37-GAPDH using the baculovirus-insect cell system. Large amounts of soluble rSm37-GAPDH with enzymatic activity were obtained. Most sera from individuals living in an area endemic for S. mansoni recognised the rSm37 molecule and inhibited its catalytic activity.
Authors:
L Argiro; C Doerig; S Liabeuf; A Bourgois; J L Romette
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  68     ISSN:  0006-3592     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-06-20     Completed Date:  2000-06-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  136-41     Citation Subset:  IM    
Copyright Information:
Copyright 2000 John Wiley & Sons, Inc.
Affiliation:
Laboratory of Immunology and Genetics of Parasitic Diseases, INSERM U399, Faculté de Médecine, 27 Blvd. Jean Moulin, 13385 Marseille Cedex 5, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Baculoviridae / genetics
Blotting, Western
Cells, Cultured
Chromatography, Gel
DNA, Recombinant / genetics
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Glyceraldehyde-3-Phosphate Dehydrogenases / biosynthesis*,  immunology,  isolation & purification
Humans
Immune Sera
Lepidoptera / cytology
Recombinant Proteins / biosynthesis
Schistosoma mansoni / enzymology*,  immunology
Schistosomiasis / immunology,  prevention & control*
Vaccines, Synthetic / biosynthesis
Chemical
Reg. No./Substance:
0/DNA, Recombinant; 0/Immune Sera; 0/Recombinant Proteins; 0/Vaccines, Synthetic; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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