Document Detail


Production of (R)-ethyl-3,4-epoxybutyrate by newly isolated Acinetobacter baumannii containing epoxide hydrolase.
MedLine Citation:
PMID:  18301887     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several new microorganisms have been isolated from soil samples with high epoxide hydrolase activity toward ethyl 3,4-epoxybutyrate. Screening was performed by enrichment culture on alkenes as sole carbon source, followed by chiral gas chromatography. Eight strains were discovered with enantioselectivity from moderate to high level and identified as bacterial and yeast species. Cells were cultivated under aerobic condition at 30 degrees C using glucose as carbon source and resting cells were used as biocatalysts for kinetic resolution of ethyl 3,4-epoxybutyrate. Among isolated microorganisms, Acinetobacter baumannii showed highest enantioselectivity for (S)-enantiomer, resulting in (R)-ethyl-3,4-epoxybutyrates (>99%ee, 46% yield). It is the first report on the fact that epoxide hydrolases originating from bacterial species of A. baumannii was applied to kinetic resolution of ethyl 3,4-epoxybutyrate in order to obtain enantiopure high-value-added (R)-ethyl-3,4-epoxybutyrate.
Authors:
Won Jae Choi; Sze Min Puah; Li Ling Tan; Soon Seng Ng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-27
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  79     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-14     Completed Date:  2008-07-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  61-7     Citation Subset:  IM    
Affiliation:
Institute of Chemical and Engineering Sciences, A*STAR (Agency for Science, Technology and Research), Jurong Island, Singapore. choi_won_jae@ices.a-star.edu.sg
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MeSH Terms
Descriptor/Qualifier:
Acinetobacter baumannii / enzymology*,  isolation & purification*
Alkenes / metabolism
Butyrates / metabolism
Chromatography, Gas
Culture Media
Epoxide Hydrolases / metabolism*
Epoxy Compounds / isolation & purification,  metabolism*
Fermentation
Hydrolysis
Kinetics
Soil Microbiology
Substrate Specificity
Chemical
Reg. No./Substance:
0/Alkenes; 0/Butyrates; 0/Culture Media; 0/Epoxy Compounds; 0/ethyl-3,4-epoxybutyrate; EC 3.3.2.-/Epoxide Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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