Document Detail


Production of a Mating Inhibitor by Ustilago hordei.
MedLine Citation:
PMID:  18944927     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
ABSTRACT Ustilago hordei, the cause of barley covered smut, was found to produce a factor that inhibited its own mating. The mating inhibition factor (MIF) specifically inhibited mating of U. hordei and other Ustilago spp., but not teliospore germination or sporidial growth. MIF did prevent teliospore germination of Tilletia caries and T. contraversa. MIF was found at low levels in culture supernatants of either mating type of U. hordei grown separately, but at higher levels when both mating types were grown together, in the supernatants of MAT-1 mating type cells transformed with the MAT-1 pheromone gene mfa1 and of MAT-2 cells transformed with either mfa1 or the MAT-1 pheromone receptor gene pra1. Diploid cells produced no detectable inhibitor, nor did MAT-1 cells with a disrupted mating type locus that deleted both mfa1 and pra1. MIF production was restored when mfa1, but not pra1, was added back to the MAT-1Delta cells. MIF activity was altered by protease treatment. Highly purified MIF from MAT-1 cells contained cysteine methyl ester, farnesyl cysteine, farnesyl cysteine methyl ester, and a dodecapeptide with a mass consistent with that of MAT-1 pheromone lacking the terminal cysteine. Since smut fungi must first mate to become pathogenic, mating inhibition has the potential to be an effective method of disease control for these pathogens.
Authors:
J E Sherwood; P J Kosted; C M Anderson; S A Gerhardt
Related Documents :
40957 - Sucrose transport by the escherichia coli lactose carrier.
21435237 - Antiviral propierties of 5,5'-dithiobis-2-nitrobenzoic acid and bacitracin against t-tr...
2275537 - Survival in seawater of escherichia coli cells grown in marine sediments containing gly...
15337757 - Murine wnt-1 with an internal c-myc tag recombinantly produced in escherichia coli can ...
7923557 - Relationship of cell survival, drug dose, and drug uptake after 1-o-octadecyl-2-o-methy...
820057 - Separation of mouse epidermal basal and differentiating cells for microflow fluorometri...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Phytopathology     Volume:  88     ISSN:  0031-949X     ISO Abbreviation:  Phytopathology     Publication Date:  1998 May 
Date Detail:
Created Date:  2008-10-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9427222     Medline TA:  Phytopathology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  456-64     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Compost and compost water extract-induced systemic acquired resistance in cucumber and Arabidopsis.
Next Document:  Isolation, Characterization, and Distribution of a Biocontrol Fungus from Cysts of Heterodera glycin...