Document Detail


Production of L. pneumophila Monospecies Biofilms in a Low-Nutrient-Concentration Medium.
MedLine Citation:
PMID:  23150398     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In aquatic environments such as water distribution systems, Legionella pneumophila persistence is -correlated to the presence of a biofilm. The method described here permits the formation of a monospecies L. pneumophila biofilm in microplates, enabling the screening of multiple parameters. The culture medium used has a low nutrient concentration compared to the classical Buffered Yeast Extract culture medium. Hence, bacterial growth occurs in the sessile phase, allowing the formation of three-dimensional structures.
Authors:
Sophie Pécastaings; Christine Roques
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Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  954     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  219-224     Citation Subset:  -    
Affiliation:
Faculté de Pharmacie, Laboratoire de Génie Chimique, BioSym Department, Université de Toulouse, Toulouse, France, sophie@pecastaings.net.
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