| Production of IL-10 and IL-12 by antigen-presenting cells in periapical lesions. | |
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MedLine Citation:
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PMID: 20819132 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Interferon-γ (IFN-γ) plays an important role in the pathogenesis of periapical lesions. Its expression is up-regulated by interleukin (IL)-12) and down-regulated by IL-10. The aim of this work was to study the cellular source of these cytokines and their mutual interactions in human periapical lesions. METHODS: Mononuclear cells, macrophages and dendritic cells were isolated from periapical lesions using plastic adherence and osmotic gradients. Cytokines were measured in culture supernatants by a microbeads fluorescence assay. Phenotypic characteristics of cells were studied by immunocytochemistry, whereas allostimulatory activity of antigen-presenting cells was tested using a mixed leukocyte reaction. RESULTS: We observed the positive correlations between the levels of IL-12 and IFN-γ as well as IL-12 and IL-10 in cultures of mononuclear cells. As IL-10 and IL-12 are produced by dendritic cells and activated macrophages, we examined their contribution to the production of these cytokines. Macrophages, CD14(+) adherent cells, produced high levels of IL-10 and very low levels of IL-12. In contrast, non-adherent, strongly HLA-DR(+) dendritic cells, potent stimulators of the alloreactive T-cell response, produced low levels of IL-10 and moderate levels of IL-12. Dendritic cells stimulated the production of IFN-γ by allogeneic CD4(+) T cells. In contrast, the level of IFN-γ was significantly decreased and the production of IL-10 was enhanced by addition of macrophages to the culture system. CONCLUSION: Our results suggest that a fine balance between the production of IL-10 and IL-12 by different antigen-presenting cells, through IFN-γ, may control the course of chronic inflammation in periapical lesions. |
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Authors:
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Miodrag Colic; Dragan Gazivoda; Sasa Vasilijic; Dragana Vucevic; Aleksandra Lukic |
Publication Detail:
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Type: Journal Article Date: 2010-08-31 |
Journal Detail:
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Title: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology Volume: 39 ISSN: 1600-0714 ISO Abbreviation: J. Oral Pathol. Med. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-04 Completed Date: 2011-01-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8911934 Medline TA: J Oral Pathol Med Country: Denmark |
Other Details:
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Languages: eng Pagination: 690-6 Citation Subset: D; IM |
Copyright Information:
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© 2010 The Authors. Journal compilation © 2010 John Wiley & Sons A/S. |
Affiliation:
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Institute for Medical Research, Military Medical Academy, Belgrade, Serbia. vmaimi@eunet.rs |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Antigen-Presenting Cells / metabolism* CD4-Positive T-Lymphocytes / metabolism Cells, Cultured Coculture Techniques Culture Media, Conditioned Dendritic Cells / metabolism Flow Cytometry Fluorescent Antibody Technique Humans Immunophenotyping Interferon-gamma / biosynthesis* Interleukin-10 / biosynthesis* Interleukin-12 / biosynthesis* Macrophages / metabolism Middle Aged Periapical Periodontitis / immunology*, metabolism Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 130068-27-8/Interleukin-10; 187348-17-0/Interleukin-12; 82115-62-6/Interferon-gamma |
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