Document Detail


Production of Human Milk Fat Analogue Containing Docosahexaenoic and Arachidonic Acids.
MedLine Citation:
PMID:  22497589     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Human milk fat (HMF) analogue containing docosahexaenoic acid (DHA) and arachidonic acid (ARA) at sn-1,3 positions and palmitic acid (PA) at sn-2 position was produced. Novozym® 435 lipase was used to produce palmitic acid-enriched hazelnut oil (EHO). EHO was then used to produce the final structured lipid (SL) through interesterification reactions using Lipozyme® RM IM. Reaction variables for 3h reactions were temperature, substrate mole ratio, and ARASCO®/DHASCO® (A:D) ratio. After statistical analysis of DHA, ARA, total PA, and PA content at sn-2 position, a large scale production was performed at 60ºC, 3:2 A:D ratio, and 1:0.1 substrate mole ratio. For the SL, those results were determined as 57.3±0.4, 2.7±0.0, 2.4±0.1%, and 66.1±2.2%, respectively. Tocopherol contents were 84, 19, 85, and 23 μg/g oil for α-, β-, γ-, and δ-tocopherol. Melting range of SL was narrower than that of EHO. Oxidative stability index (OSI) value of SL (0.80 h) was similar to EHO (0.88 h). This SL can be used in infant formulas to provide the benefits of ARA and DHA.
Authors:
Dilek Turan; Nese Sahin Yesilcubuk; Casimir C Akoh
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-12
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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