Document Detail

Production of D-tagatose at high temperatures using immobilized Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana.
MedLine Citation:
PMID:  17206372     Owner:  NLM     Status:  MEDLINE    
Escherichia coli cells expressing L-arabinose isomerase from Thermotoga neapolitana (TNAI) were immobilized in calcium alginate beads. The resulting cell reactor (2.4 U, t (1/2) = 43 days at 70 degrees C) in a continuous recycling mode at 70 degrees C produced 49 and 38 g D-tagatose/l from 180 and 90 g D-galactose/l, respectively, within 12 h.
Young-Ho Hong; Dong-Woo Lee; Sang-Jae Lee; Eun-Ah Choe; Seong-Bo Kim; Yoon-Hee Lee; Chan-Ick Cheigh; Yu-Ryang Pyun
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-06
Journal Detail:
Title:  Biotechnology letters     Volume:  29     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-07     Completed Date:  2007-09-04     Revised Date:  2008-10-07    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  569-74     Citation Subset:  IM    
Department of Biotechnology, Yonsei University, Seoul, Korea.
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MeSH Terms
Arabinose / genetics,  metabolism*
Cell Culture Techniques / methods
Escherichia coli / genetics,  metabolism*
Hexoses / biosynthesis*,  genetics
Isomerases / genetics,  metabolism*
Protein Engineering / methods*
Recombinant Proteins / metabolism
Sweetening Agents / metabolism*
Thermotoga neapolitana / enzymology*,  genetics
Reg. No./Substance:
0/Hexoses; 0/Recombinant Proteins; 0/Sweetening Agents; 147-81-9/Arabinose; 17598-81-1/tagatose; EC 5.-/Isomerases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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