Document Detail


Production of D-lactic acid by Corynebacterium glutamicum under oxygen deprivation.
MedLine Citation:
PMID:  18188553     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In mineral salts medium under oxygen deprivation, Corynebacterium glutamicum exhibits high productivity of L-lactic acid accompanied with succinic and acetic acids. In taking advantage of this elevated productivity, C. glutamicum was genetically modified to produce D-lactic acid. The modification involved expression of fermentative D-lactate dehydrogenase (D-LDH)-encoding genes from Escherichia coli and Lactobacillus delbrueckii in L-lactate dehydrogenase (L-LDH)-encoding ldhA-null C. glutamicum mutants to yield strains C. glutamicum DeltaldhA/pCRB201 and C. glutamicum DeltaldhA/pCRB204, respectively. The productivity of C. glutamicum DeltaldhA/pCRB204 was fivefold higher than that of C. glutamicum DeltaldhA/pCRB201. By using C. glutamicum DeltaldhA/pCRB204 cells packed to a high density in mineral salts medium, up to 1,336 mM (120 g l(-1)) of D-lactic acid of greater than 99.9% optical purity was produced within 30 h.
Authors:
Shohei Okino; Masako Suda; Keitaro Fujikura; Masayuki Inui; Hideaki Yukawa
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Publication Detail:
Type:  Journal Article     Date:  2008-01-10
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  78     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-13     Completed Date:  2008-05-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  449-54     Citation Subset:  IM    
Affiliation:
Research Institute of Innovative Technology for the Earth, 9-2 Kizugawadai, Kizugawa, Kyoto, 619-0292, Japan. mmg-lab@rite.or.jp
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism
Corynebacterium glutamicum / metabolism*
Escherichia coli Proteins / genetics,  metabolism
Gene Expression Regulation, Bacterial
Genetic Engineering
L-Lactate Dehydrogenase / genetics,  metabolism*
Lactic Acid / analogs & derivatives,  metabolism*
Oxygen / metabolism*
Plasmids
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Escherichia coli Proteins; 50-21-5/Lactic Acid; 7782-44-7/Oxygen; EC 1.1.1.27/L-Lactate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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