| Production of D-lactic acid by Corynebacterium glutamicum under oxygen deprivation. | |
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MedLine Citation:
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PMID: 18188553 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In mineral salts medium under oxygen deprivation, Corynebacterium glutamicum exhibits high productivity of L-lactic acid accompanied with succinic and acetic acids. In taking advantage of this elevated productivity, C. glutamicum was genetically modified to produce D-lactic acid. The modification involved expression of fermentative D-lactate dehydrogenase (D-LDH)-encoding genes from Escherichia coli and Lactobacillus delbrueckii in L-lactate dehydrogenase (L-LDH)-encoding ldhA-null C. glutamicum mutants to yield strains C. glutamicum DeltaldhA/pCRB201 and C. glutamicum DeltaldhA/pCRB204, respectively. The productivity of C. glutamicum DeltaldhA/pCRB204 was fivefold higher than that of C. glutamicum DeltaldhA/pCRB201. By using C. glutamicum DeltaldhA/pCRB204 cells packed to a high density in mineral salts medium, up to 1,336 mM (120 g l(-1)) of D-lactic acid of greater than 99.9% optical purity was produced within 30 h. |
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Authors:
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Shohei Okino; Masako Suda; Keitaro Fujikura; Masayuki Inui; Hideaki Yukawa |
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Publication Detail:
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Type: Journal Article Date: 2008-01-10 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 78 ISSN: 0175-7598 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-02-13 Completed Date: 2008-05-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 449-54 Citation Subset: IM |
Affiliation:
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Research Institute of Innovative Technology for the Earth, 9-2 Kizugawadai, Kizugawa, Kyoto, 619-0292, Japan. mmg-lab@rite.or.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics,
metabolism Corynebacterium glutamicum / metabolism* Escherichia coli Proteins / genetics, metabolism Gene Expression Regulation, Bacterial Genetic Engineering L-Lactate Dehydrogenase / genetics, metabolism* Lactic Acid / analogs & derivatives, metabolism* Oxygen / metabolism* Plasmids |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Escherichia coli Proteins; 50-21-5/Lactic Acid; 7782-44-7/Oxygen; EC 1.1.1.27/L-Lactate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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