| Production and characterization of N- and C-terminally truncated Mtx2: a mosquitocidal toxin from Bacillus sphaericus. | |
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MedLine Citation:
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PMID: 20411263 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Mosquitocidal toxin 2 (Mtx2) is a mosquito-larvicidal protein produced during vegetative stage of Bacillus sphaericus. The toxin consists of 292 amino acids and has a molecular weight of 31.8 kDa. To determine the active core region of the toxin, amino acids at N- and C-termini were sequentially removed. Deletion up to 23 amino acids from the N-terminus (Met1-Tyr23) did not significantly affect protein production and the toxin activity, whereas removal of 26 amino acids from the N-terminus (Met1-Lys26) completely abolished toxicity even though the protein production remained unchanged. Deletion of only 5 amino acids from the C-terminal end yielded the protein that could not be solubilized and rendered the toxin inactive. The results demonstrated that the C-terminal end of Mtx2 is required for proper folding and toxicity. Amino acids at the N-terminus up to Tyr23 did not play a significant role in protein production and toxicity whereas amino acids between Thr24 and Lys26 are required for full toxicity. |
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Authors:
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Kulwadee Phannachet; Ponlawoot Raksat; Thidarat Limvuttegrijeerat; Boonhiang Promdonkoy |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-22 |
Journal Detail:
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Title: Current microbiology Volume: 61 ISSN: 1432-0991 ISO Abbreviation: Curr. Microbiol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7808448 Medline TA: Curr Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 549-53 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, Faculty of Science and Technology, Thammasat University, 99 Pahonyothin Road, Klong 1, Klong Luang, Pathumthani, 12120, Thailand. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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