Document Detail


Production of C21 steroids in rat fetal ovaries.
MedLine Citation:
PMID:  2845196     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Female gonads of fetal and neonatal rats were cultured for 24 h in medium 199 supplemented with dibutyryl cyclic AMP (dcAMP), cycloheximide, spironolactone (an inhibitor of 17 alpha-hydroxylase) or hydroxylated cholesterol derivatives. Radioimmunoassays of pregnenolone (P5) and progesterone (P4) were performed in the media. In control medium, progesterone production developed during the second week of life whereas ovaries treated with dcAMP (1 mM) produced progesterone as early as day 14.5 of gestation. The effect of the nucleotide was reversible and the lag period of responsiveness was 12 h. Cycloheximide (1 microgram/ml) added with dcAMP completely inhibited the production of progesterone. Addition of spironolactone to the basal medium was without effect but significantly increased P5 and P4 productions in the presence of dcAMP. The inhibitory effects of spironolactone and trilostane on steroidogenesis were tested in fetal testis. Lastly, the dcAMP effect could not be mimicked with hydroxylated cholesterol derivatives (20 microM). However, an additive effect of 22-hydroxycholesterol was observed in the presence of the nucleotide. These results indicate that the in vitro production of P5 and P4 by fetal ovaries in the presence of dcAMP suggests that dcAMP induces enzymes involved in C21 steroid synthesis. Levels of action of the nucleotide are discussed.
Authors:
V Rouiller; M N Gangnerau; R Picon
Related Documents :
21376476 - Hla-g and pregnancy adverse outcomes.
21241516 - The effects of pre-pregnancy body mass index and gestational weight gain on perinatal o...
21307866 - Clinical application of fetal sex determination using cell-free fetal dna in pregnant c...
21269546 - Maternal plasma fatty acid composition and pregnancy outcome in adolescents.
3378946 - Use of birth fluids and cervical stimulation in lamb fostering.
24735486 - Prenatal diagnosis of amniotic band syndrome - risk factors and ultrasonic signs.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of steroid biochemistry     Volume:  31     ISSN:  0022-4731     ISO Abbreviation:  J. Steroid Biochem.     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1988-11-22     Completed Date:  1988-11-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0260125     Medline TA:  J Steroid Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  447-52     Citation Subset:  IM    
Affiliation:
Laboratoire de Physiologie du Développement, U.A. C.N.R.S. no. 307, Université Paris 7, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
3-Hydroxysteroid Dehydrogenases / antagonists & inhibitors
Animals
Bucladesine / pharmacology
Cycloheximide / pharmacology
Female
Hydroxycholesterols / pharmacology
Kinetics
Organ Culture Techniques
Ovary / drug effects,  embryology*,  metabolism
Pregnenolone / biosynthesis*
Progesterone / biosynthesis*
Rats
Rats, Inbred Strains
Spironolactone / pharmacology
Steroid 17-alpha-Hydroxylase / antagonists & inhibitors
Chemical
Reg. No./Substance:
0/Hydroxycholesterols; 145-13-1/Pregnenolone; 362-74-3/Bucladesine; 52-01-7/Spironolactone; 57-83-0/Progesterone; 66-81-9/Cycloheximide; EC 1.1.-/3-Hydroxysteroid Dehydrogenases; EC 1.14.99.9/Steroid 17-alpha-Hydroxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inhibition of catechol estrogen formation in rat liver microsomes by hormonal steroids and related c...
Next Document:  Radiotherapy and L-triiodothyronine in the treatment of high-grade astrocytoma.