| Production of Bovine Hand-Made Cloned Embryos by Zygote-Oocyte Cytoplasmic Hemi-complementation. | |
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MedLine Citation:
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PMID: 21241164 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Abstract The aim of this study was to evaluate the effect of the cytoplast type and activation process on development of cloned embryos. Bovine oocytes (MII) or zygotes at the one-cell stage (IVF) were manually bisected and segregated in MII or IVF hemi-cytoplasts or hemi-karyoplasts. Adult skin cells from a bovine female were used as nucleus donors (SC). Experimental groups were composed of IVF embryos; parthenogenetic embryos; hand-made cloned (HMC) embryos; and reconstructed HMC embryos using IVF hemi-cytoplast + MII hemi-cytoplast + SC (G-I); IVF hemi-cytoplast + IVF hemi-cytoplast + SC (G-II); MII hemi-cytoplast + IVF hemi-karyoplast (G-III); and IVF hemi-cytoplast + IVF hemi-karyoplast (G-IV). Embryos from G-I to G-IV were allocated to subgroups as sperm-activated (SA) or were further chemically activated (SA + CA). Embryos from all groups and subgroups were in vitro cultured in the WOW system. Blastocyst development in subgroup G-I SA (28.2%) was similar to IVF (27.0%) and HMC (31.4%) controls, perhaps due to a to a more suitable activation process and/or better complementation of cytoplasmic reprogramming factors, with the other groups and subgroups having lower levels of development. No blastocyst development was observed when using IVF hemi-karyoplasts (G-III and G-IV), possibly due to the manipulation process during a sensitive biological period. In summary, the presence of cytoplasmic factors from MII hemi-oocytes and the sperm activation process from hemi-zygotes appear to be necessary for adequate in vitro development, as only the zygote-oocyte hemi-complementation was as efficient as controls for the generation of bovine cloned blastocysts. |
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Authors:
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Joana Claudia Mezzalira; Lain Uriel Ohlweiler; Renato Pereira da Costa Gerger; Renata Casali; Fabiano Koerich Vieira; Carlos Eduardo Ambrósio; Maria Angélica Miglino; José Luiz Rodrigues; Alceu Mezzalira; Marcelo Bertolini |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-1-17 |
Journal Detail:
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Title: Cellular reprogramming Volume: - ISSN: 2152-4998 ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-1-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101528176 Medline TA: Cell Reprogram Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1 Animal Reproduction Laboratory, Center of Agronomy and Veterinary Sciences (CAV), Santa Catarina State University (UDESC) , Santa Catarina, Brazil . |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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