Document Detail

Production and Application of Long dsRNA in Mammalian Cells.
MedLine Citation:
PMID:  23027058     Owner:  NLM     Status:  In-Data-Review    
Double-stranded RNA (dsRNA) is involved in different biological processes. At least three different pathways can respond to dsRNA in mammals. One of these pathways is RNA interference (RNAi) where long dsRNA induces sequence-specific degradation of transcripts carrying sequences complementary to dsRNA. Long dsRNA is also a potent trigger of the interferon pathway, a sequence-independent response that leads to global suppression of translation and global RNA degradation. In addition, dsRNA can be edited by adenosine deamination, which may result in nuclear retention and degradation of dsRNA or in alteration of RNA coding potential. Here, we provide a technical review summarizing different strategies of long dsRNA usage. While the review is largely focused on long dsRNA-induced RNAi in mammalian cells, it also provides helpful information on both the in vitro production and in vivo expression of dsRNAs. We present an overview of currently available vectors for dsRNA expression and provide the latest update on oocyte-specific transgenic RNAi approaches.
Katerina Chalupnikova; Jana Nejepinska; Petr Svoboda
Related Documents :
9890418 - Molecular genotyping of hiv-1 in 61 patients with aids from lomé, togo.
18370038 - Detection and identification of the h5 hemagglutinin subtype by real-time rt-pcr.
18753218 - Classification of hepatitis c virus and human immunodeficiency virus-1 sequences with t...
8892038 - Initial characterization of viral sequences from a shiv-inoculated pig-tailed macaque t...
2497298 - Conserved lipoprotein h.8 of pathogenic neisseria consists entirely of pentapeptide rep...
1420318 - Cloning and 5'-flanking sequence of a rat cholesterol 7 alpha-hydroxylase gene.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  942     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  291-314     Citation Subset:  IM    
Institute of Molecular Genetics AS CR, Prague, Czech Republic,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Design and Chemical Modification of Synthetic Short shRNAs as Potent RNAi Triggers.
Next Document:  Design of RNAi Reagents for Invertebrate Model Organisms and Human Disease Vectors.