| Production of 4-ethylphenol from 4-hydroxycinnamic acid by Lactobacillus sp. isolated from a swine waste lagoon. | |
| | |
MedLine Citation:
|
PMID: 20028439 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
AIMS: To determine the source material and the responsible micro-organisms of 4-ethylphenol production in swine lagoon sediment slurries. METHODS AND RESULTS: Swine lagoon sediment was blended and incubated with tryptone-yeast extract broth containing 10 mmol l(-1) each of 4-hydroxycinnamic as well as other phenolics. 4-Ethylphenol was only produced from 4-hydroxycinnamic acid. Denaturing gradient gel electrophoresis analysis indicated that the microbial community was substantially altered from inclusion of 4-hydroxycinnamic acid. Serial dilutions and selective plating were employed to isolate a culture capable of 4-hydroxycinnamic acid conversion to 4-ethylphenol. Morphological and ribosomal gene analysis indicated the isolate to be a Lactobacillus sp., Lactobacillus sp. pep8 converted 4-vinylphenol, but not 4-hydroxy-3-phenylpropionate, to 4-ethylphenol and did not convert 4-hydroxy-3-methoxycinnamic acid to 4-ethyl-2-methoxyphenol. A p-coumaric acid decarboxylase (pdc) gene identical to Lact. plantarum pdc genes was cloned from Lactobacillus sp. pep8. CONCLUSIONS: Swine lagoon sediments produce 4-ethylphenol from 4-hydroxycinnamic acid due, in part, to the activity of Lactobacillus spp. SIGNIFICANCE AND IMPACT OF THE STUDY: 4-Ethylphenol, a malodourant of swine and beef wastes, is generated from plant materials by indigenous lactobacilli suggesting that altering the amount dietary of plant material may influence levels of 4-ethylphenol in the wastes. |
| | |
Authors:
|
D Kridelbaugh; S Hughes; T Allen; K C Doerner |
Related Documents
:
|
22040569 - Inhibition of nitric oxide synthase lowers fatty acid oxidation in preeclampsia-like mi... 18161559 - Photocatalytic ozonation of phenolic wastewaters: syringic acid, tyrosol and gallic acid. 15355309 - The predominant polyphenol in the leaves of the resurrection plant myrothamnus flabelli... 12520369 - Effect of plant growth-promoting rhizobacteria and culture filtrate of sclerotium rolfs... 15678719 - Quality evaluation of honey harvested from selected areas in tanzania with special emph... 14690369 - Characterization of metabolites of hydroxycinnamates in the in vitro model of human sma... 7726379 - A new patient with alpha-ketoglutaric aciduria and progressive extrapyramidal tract dis... 6377109 - Decreased labeling of amino acids by inhibition of the utilization of [3h, 14c]glucose ... 4287769 - Synthesis and characterization of 1- and 2-monoglycerides of anteiso fatty acids. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2009-11-30 |
Journal Detail:
|
Title: Journal of applied microbiology Volume: 109 ISSN: 1365-2672 ISO Abbreviation: J. Appl. Microbiol. Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-10-13 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9706280 Medline TA: J Appl Microbiol Country: England |
Other Details:
|
Languages: eng Pagination: 190-8 Citation Subset: IM |
Copyright Information:
|
Journal compilation © 2009 The Society for Applied Microbiology. No claim to original US Government works. |
Affiliation:
|
Department of Biology, Western Kentucky University, Bowling Green, KY 42101-1080, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Grant Support | |
ID/Acronym/Agency:
|
2 P20 RR-16481/RR/NCRR NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: High hydrostatic pressure resistance of Campylobacter jejuni after different sublethal stresses.
Next Document: Application of quantitative reverse-transcription PCR (qRT-PCR) for the determination of the total v...