Document Detail


Production of 1,25 dihydroxyvitamin D3 by perfused pig skin.
MedLine Citation:
PMID:  8176265     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Keratinocytes produce large amounts of 1,25 dihydroxyvitamin D3 (1,25(OH)2D3) in vitro. 1,25(OH)2D3 is detectable in anephric humans and pigs and can be increased to near-normal levels by vitamin D or 250HD, indicating an extrarenal source. To determine whether the skin is one of these extrarenal sources, we perfused isolated flaps of porcine skin for 8 h with 250HD3 in serum-free medium at 1 ml/min, collecting the venous effluent as 15-min samples. The samples were extracted and the vitamin D metabolites purified by high-performance liquid chromatography and assayed by competitive protein-binding techniques. Production of 1,25(OH)2D3 continued for the duration of the perfusion, tending to increase in the last 2 hours. The amount of 1,25(OH)2D3 produced varied both with time in the same pig skin and between pig skins; maximum production of 1,25(OH)2D3 in these experiments was 8 pg/min. 24,25(OH)2D3 production was higher than 1,25(OH)2D3 production, reaching a maximum rate of 180 pg/min. Considering that the production rate of 1,25(OH)2D3 in humans is 1.25 ng/min and that a 48-cm2 skin flap represents 1/350 the surface area of a human, and assuming that human and pig skin make 1,25(OH)2D3 at comparable rates, one can determine that the skin has the potential to maintain near-normal levels of 1,25(OH)2D3 in the absence of kidneys when provided with adequate substrate.
Authors:
D D Bikle; B P Halloran; J E Riviere
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of investigative dermatology     Volume:  102     ISSN:  0022-202X     ISO Abbreviation:  J. Invest. Dermatol.     Publication Date:  1994 May 
Date Detail:
Created Date:  1994-06-07     Completed Date:  1994-06-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0426720     Medline TA:  J Invest Dermatol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  796-8     Citation Subset:  IM    
Affiliation:
Endocrine Research Unit, Veterans Administration Medical Center, San Francisco, California 94121.
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MeSH Terms
Descriptor/Qualifier:
24,25-Dihydroxyvitamin D 3 / biosynthesis
Animals
Calcitriol / biosynthesis*
Chromatography
Culture Media, Serum-Free
Perfusion
Skin / metabolism*
Swine
Time Factors
Grant Support
ID/Acronym/Agency:
AR38386/AR/NIAMS NIH HHS; AR39448/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media, Serum-Free; 32222-06-3/Calcitriol; 40013-87-4/24,25-Dihydroxyvitamin D 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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