Document Detail


Production of (10E,12Z)-conjugated linoleic acid in yeast and tobacco seeds.
MedLine Citation:
PMID:  16324883     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The polyenoic fatty acid isomerase from Propioniumbacterium acnes (PAI) was expressed in E. coli and biochemically characterized. PAI catalyzes the isomerization of a methylene-interrupted double bond system to a conjugated double bond system, creating (10E,12Z)-conjugated linoleic acid (CLA). PAI accepted a wide range of free polyunsaturated fatty acids as substrates ranging from 18:2 fatty acids to 22:6, converting them to fatty acids with two or three conjugated double bonds. For expression of PAI in yeast the PAI-sequence encoding 20 N-terminal amino acid residues was altered for optimal codon usage, yielding codon optimized PAI (coPAI). The percentage of 10,12-CLA of total esterified fatty acids was 8 times higher in yeast transformed with coPAI than in cells transformed with PAI. CLA was detected in amounts up to 5.7% of total free fatty acids in yeast transformed with coPAI but none was detected in yeast transformed with PAI. PAI or coPAI under the control of the constitutive CaMV 35S promoter or the seed-specific USP promoter was transformed into tobacco plants. CLA was only detected in seeds in coPAI-transgenic plants. The amount of CLA detected in esterified fatty acids was up to 0.3%, in free fatty acids up to 15%.
Authors:
Ellen Hornung; Claudia Krueger; Christian Pernstich; Martijn Gipmans; Andrea Porzel; Ivo Feussner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-11-18
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1738     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2006-01-27     Completed Date:  2006-03-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  105-14     Citation Subset:  IM    
Affiliation:
Georg-August-University Göttingen, Albrecht-von-Haller-Institute for Plant Sciences, Department of Plant Biochemistry, Justus-von-Liebig-Weg 11, D-37077 Göttingen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Actinomycetales / enzymology,  genetics
Bacterial Proteins / genetics,  metabolism
Base Sequence
Biotechnology / methods
Carbon-Carbon Double Bond Isomerases / genetics,  metabolism*
Codon
Hydrogen-Ion Concentration
Linoleic Acids, Conjugated / biosynthesis*,  metabolism
Molecular Sequence Data
Promoter Regions, Genetic
Recombinant Proteins / genetics,  metabolism
Saccharomyces cerevisiae / genetics,  metabolism*
Seeds / genetics,  metabolism*
Substrate Specificity
Tobacco / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Codon; 0/Linoleic Acids, Conjugated; 0/Recombinant Proteins; EC 5.3.3.-/Carbon-Carbon Double Bond Isomerases; EC 5.3.3.-/polyenoic fatty acid isomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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