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Production of 10-hydroxystearic acid from oleic acid by whole cells of recombinant Escherichia coli containing oleate hydratase from Stenotrophomonas maltophilia.
MedLine Citation:
PMID:  22261174     Owner:  NLM     Status:  Publisher    
A putative fatty acid hydratase from Stenotrophomonas maltophilia was cloned and expressed in Escherichia coli. The recombinant enzyme showed the highest hydration activity for oleic acid among the fatty acids tested, indicating that the enzyme is an oleate hydratase. The optimal conditions for the production of 10-hydroxystearic acid from oleic acid using whole cells of recombinant E. coli containing the oleate hydratase were pH 6.5, 35°C, 0.05% (w/v) Tween 40, 10gl(-1) cells, and 50gl(-1) oleic acid. Under these conditions, whole recombinant cells produced 49gl(-1) 10-hydroxystearic acid for 4h, with a conversion yield of 98% (w/w), a volumetric productivity of 12.3gl(-1)h(-1), and a specific productivity of 1.23gg-cells(-1)h(-1), which were 18%, 2.5-, and 2.5-fold higher than those of whole wild-type S. maltophilia cells, respectively. This is the first report of 10-hydroxystearic acid production using recombinant cells and the concentration and productivity are the highest reported thus far among cells.
Young-Chul Joo; Eun-Sun Seo; Yeong-Su Kim; Kyoung-Rok Kim; Jin-Byung Park; Deok-Kun Oh
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-12
Journal Detail:
Title:  Journal of biotechnology     Volume:  -     ISSN:  1873-4863     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.
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