| Production of 10-hydroxystearic acid from oleic acid and olive oil hydrolyzate by an oleate hydratase from Lysinibacillus fusiformis. | |
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MedLine Citation:
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PMID: 22189865 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A recombinant enzyme from Lysinibacillus fusiformis was expressed, purified, and identified as an oleate hydratase because the hydration activity of the enzyme was the highest for oleic acid (with a k (cat) of 850 min(-1) and a K (m) of 540 μM), followed by palmitoleic acid, γ-linolenic acid, linoleic acid, myristoleic acid, and α-linolenic acid. The optimal reaction conditions for the enzymatic production of 10-hydroxystearic acid were pH 6.5, 35 °C, 4% (v/v) ethanol, 2,500 U ml(-1) (8.3 mg ml(-1)) of enzyme, and 40 g l(-1) oleic acid. Under these conditions, 40 g l(-1) (142 mM) oleic acid was converted into 40 g l(-1) (133 mM) 10-hydroxystearic acid for 150 min, with a molar yield of 94% and a productivity of 16 g l(-1) h(-1), and olive oil hydrolyzate containing 40 g l(-1) oleic acid was converted into 40 g l(-1) 10-hydroxystearic acid for 300 min, with a productivity of 8 g l(-1) h(-1). |
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Authors:
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Bi-Na Kim; Young-Chul Joo; Yeong-Su Kim; Kyoung-Rok Kim; Deok-Kun Oh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-12-22 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 95 ISSN: 1432-0614 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-07-26 Completed Date: 2012-11-26 Revised Date: 2012-11-27 |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 929-37 Citation Subset: IM |
Affiliation:
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Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillaceae
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enzymology* Base Sequence Cloning, Molecular DNA Primers Electrophoresis, Polyacrylamide Gel Gas Chromatography-Mass Spectrometry Hydro-Lyases / chemistry, genetics, metabolism* Hydrogen-Ion Concentration Hydrolysis Kinetics Molecular Weight Oleic Acid / metabolism* Plant Oils / metabolism* Polymerase Chain Reaction Stearic Acids / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Plant Oils; 0/Stearic Acids; 112-80-1/Oleic Acid; 638-26-6/10-hydroxystearic acid; 8001-25-0/olive oil; EC 4.2.1.-/Hydro-Lyases |
| Comments/Corrections | |
Erratum In:
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Appl Microbiol Biotechnol. 2012 Aug;95(4):1095-6 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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