Document Detail


Prodigiosin induces caspase-9 and caspase-8 activation and cytochrome C release in Jurkat T cells.
MedLine Citation:
PMID:  12485870     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Prodigiosin (PG) is an active component of bacterial origin, with reported apoptotic effects. We examined the activation of caspases-9, -8, and -3 in PG-treated Jurkat cells in a dose-response study. These caspases were activated in apoptotic cells, as judged by the appearance of cleavage products from procaspases, and cytochrome c (cyt c) was released to the cytosol. In addition, PG induced the cleavage of poly (ADP-ribose) polymerase (PARP). This study demonstrates that the activation of caspases-9, -8, and -3 mediates PG-induced apoptosis.
Authors:
Beatriz Montaner; Ricardo Perez-Tomas
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  973     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-12-17     Completed Date:  2003-02-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  246-9     Citation Subset:  IM    
Affiliation:
Departament de Biologia Cellular i Anatomia Patològica Cancer Cell Biology Research Group, Universitat de Barcelona, Feixa Llarga s/n, E-08907 L'Hospitalet, Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / pharmacology
Caspase 8
Caspase 9
Caspases / metabolism*
Cytochrome c Group / metabolism*
Cytosol / drug effects,  metabolism
Humans
Jurkat Cells
Kinetics
Poly(ADP-ribose) Polymerases / metabolism
Prodigiosin / pharmacology*
Serratia marcescens
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Cytochrome c Group; 82-89-3/Prodigiosin; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/CASP8 protein, human; EC 3.4.22.-/CASP9 protein, human; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9; EC 3.4.22.-/Caspases

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