| Procysteine increases alcohol-depleted glutathione stores in rat plantaris following a period of abstinence. | |
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MedLine Citation:
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PMID: 20935073 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To assess the effectiveness of procysteine (PRO) supplementation provided during a period of abstinence (ABS) on alcohol-induced skeletal muscle atrophy and oxidant stress. METHODS: Age- and gender-matched Sprague-Dawley rats were fed the Lieber-DeCarli liquid diet containing either alcohol or an isocaloric substitution (control diet) for 12 week. Next, subgroups of alcohol-fed rats were fed the control diet for 2 week (ABS) supplemented with either PRO (0.35%, w/v) or vehicle. Plantaris morphology was assessed by hematoxylin and eosin staining. Total, reduced and oxidized glutathione (GSH) levels and total antioxidant potential were determined by commercially available assay kits. Antibody arrays were used to determine cytokine levels. Real-time polymerase chain reaction was used to determine gene expressions of two E3 ubiquitin ligases, atrogin-1 and muscle ring finger protein-1 (MuRF-1). RESULTS: Plantaris muscles from alcohol-fed rats displayed extensive atrophy, as well as decreased GSH levels, a trend for decreased total antioxidant potential and elevated atrogin-1 and MuRF-1 mRNA levels. GSH levels and total antioxidant potential continued to decrease during 2 weeks of ABS from alcohol, which were normalized in abstinent rats provided PRO. Gene levels of both E3 ligases returned to baseline during ABS. In parallel, plantaris cross-sectional area increased in both groups during ABS. CONCLUSIONS: PRO supplementation during ABS significantly attenuated alcohol-induced redox stress compared with untreated abstinent rats. Thus, our data may suggest that GSH restoration therapy may provide therapeutic benefits to the overall antioxidant state of skeletal muscle when prescribed in conjunction with an established detoxification program for recovering alcoholics. |
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Authors:
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Jeffrey S Otis; David M Guidot |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-10-08 |
Journal Detail:
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Title: Alcohol and alcoholism (Oxford, Oxfordshire) Volume: 45 ISSN: 1464-3502 ISO Abbreviation: Alcohol Alcohol. Publication Date: 2010 Nov-Dec |
Date Detail:
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Created Date: 2010-11-15 Completed Date: 2011-06-08 Revised Date: 2011-06-09 |
Medline Journal Info:
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Nlm Unique ID: 8310684 Medline TA: Alcohol Alcohol Country: England |
Other Details:
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Languages: eng Pagination: 495-500 Citation Subset: IM |
Affiliation:
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Division of Pulmonary, Allergy, and Critical Care Medicine, Emory University School of Medicine, Whitehead Biomedical Research Building, 615 Michael Street, Atlanta, GA 30322, USA. jsotis@emory.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alcohol Drinking
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drug therapy,
metabolism* Animals Antioxidants / pharmacology, therapeutic use Atrophy Ethanol / toxicity* Glutathione / biosynthesis, metabolism* Male Muscle, Skeletal / drug effects, metabolism*, pathology Pyrrolidonecarboxylic Acid / pharmacology*, therapeutic use Rats Rats, Sprague-Dawley Temperance* Thiazolidines / pharmacology*, therapeutic use |
| Grant Support | |
ID/Acronym/Agency:
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K01 AA017190-02/AA/NIAAA NIH HHS; P-50 AA013757/AA/NIAAA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antioxidants; 0/Thiazolidines; 19750-45-9/2-oxothiazolidine-4-carboxylic acid; 64-17-5/Ethanol; 70-18-8/Glutathione; 98-79-3/Pyrrolidonecarboxylic Acid |
| Comments/Corrections | |
Erratum In:
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Alcohol Alcohol. 2011 May-Jun;46(3):367 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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