| Processus and recessus adhaerentes: giant adherens cell junction systems connect and attract human mesenchymal stem cells. | |
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MedLine Citation:
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PMID: 17372769 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Substrate-adherent cultured cells derived from human bone marrow or umbilical cord blood ("mesenchymal stem cells") are of special interest for regenerative medicine. We report that such cells, which can display considerable heterogeneity with respect to their cytoskeletal protein complement, are often interconnected by special tentacle-like cell processes contacting one or several other cells. These processus adhaerentes, studded with many (usually small) puncta adhaerentia and varying greatly in length (up to more than 400 microm long), either contact each other in the intercellular space ("ET touches") or insert in a tight-fitting manner into deep plasma membrane invaginations (recessus adhaerentes), thus forming a novel kind of long (up to 50 microm) continuous cuff-like junction (manubria adhaerentia). The cell processes contain an actin microfilament core that is stabilized with ezrin, alpha-actinin, and myosin and accompanied by microtubules, and their adhering junctions are characterized by a molecular complement comprising the transmembrane glycoproteins N-cadherin and cadherin-11, in combination with the cytoplasmic plaque proteins alpha- and beta-catenin, together with p120(ctn), plakoglobin, and afadin. The processes are also highly dynamic and rapidly foreshorten as cell colonies approach a denser state of cell packing. These structures are obviously able to establish cell-cell connections, even over long distances, and can form deep-rooted and tight cell-cell adhesions. The possible relationship to similar cell processes in the embryonic primary mesenchyme and their potential in cell sorting and tissue formation processes in the body are discussed. |
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Authors:
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Patrick Wuchter; Judit Boda-Heggemann; Beate K Straub; Christine Grund; Caecilia Kuhn; Ulf Krause; Anja Seckinger; Wiebke K Peitsch; Herbert Spring; Anthony D Ho; Werner W Franke |
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Publication Detail:
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Type: Journal Article Date: 2007-03-20 |
Journal Detail:
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Title: Cell and tissue research Volume: 328 ISSN: 0302-766X ISO Abbreviation: Cell Tissue Res. Publication Date: 2007 Jun |
Date Detail:
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Created Date: 2007-04-30 Completed Date: 2007-09-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: Germany |
Other Details:
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Languages: eng Pagination: 499-514 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine V, University of Heidelberg, Im Neuenheimer Feld 410, 69120, Heidelberg, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adherens Junctions
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metabolism*,
ultrastructure Adult Cell Adhesion Cell Adhesion Molecules / analysis Cell Communication* Cell Count Cells, Cultured Connexins / analysis Cytoskeleton / metabolism Humans Mesenchymal Stem Cells / cytology*, metabolism*, ultrastructure Microscopy, Electron Models, Biological |
| Chemical | |
Reg. No./Substance:
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0/Cell Adhesion Molecules; 0/Connexins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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