Document Detail


Processus and recessus adhaerentes: giant adherens cell junction systems connect and attract human mesenchymal stem cells.
MedLine Citation:
PMID:  17372769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Substrate-adherent cultured cells derived from human bone marrow or umbilical cord blood ("mesenchymal stem cells") are of special interest for regenerative medicine. We report that such cells, which can display considerable heterogeneity with respect to their cytoskeletal protein complement, are often interconnected by special tentacle-like cell processes contacting one or several other cells. These processus adhaerentes, studded with many (usually small) puncta adhaerentia and varying greatly in length (up to more than 400 microm long), either contact each other in the intercellular space ("ET touches") or insert in a tight-fitting manner into deep plasma membrane invaginations (recessus adhaerentes), thus forming a novel kind of long (up to 50 microm) continuous cuff-like junction (manubria adhaerentia). The cell processes contain an actin microfilament core that is stabilized with ezrin, alpha-actinin, and myosin and accompanied by microtubules, and their adhering junctions are characterized by a molecular complement comprising the transmembrane glycoproteins N-cadherin and cadherin-11, in combination with the cytoplasmic plaque proteins alpha- and beta-catenin, together with p120(ctn), plakoglobin, and afadin. The processes are also highly dynamic and rapidly foreshorten as cell colonies approach a denser state of cell packing. These structures are obviously able to establish cell-cell connections, even over long distances, and can form deep-rooted and tight cell-cell adhesions. The possible relationship to similar cell processes in the embryonic primary mesenchyme and their potential in cell sorting and tissue formation processes in the body are discussed.
Authors:
Patrick Wuchter; Judit Boda-Heggemann; Beate K Straub; Christine Grund; Caecilia Kuhn; Ulf Krause; Anja Seckinger; Wiebke K Peitsch; Herbert Spring; Anthony D Ho; Werner W Franke
Related Documents :
17623229 - Changes of gap and tight junctions during differentiation of human nasal epithelial cel...
21227659 - Cell adhesive and growth behavior on electrospun nanofibrous scaffolds by designed mult...
16175559 - Morphology and tracer coupling pattern of alpha ganglion cells in the mouse retina.
1295729 - Reduced junctional permeability at interrhombomeric boundaries.
17957529 - Impaired gap junction formation and intercellular calcium signaling in urinary bladder ...
11847339 - Rap1 gtpase regulation of adherens junction positioning and cell adhesion.
10484529 - Expression of ncam recapitulates tubulogenic development in kidneys recovering from acu...
8818009 - Novel diffusion cell for in vitro transdermal permeation, compatible with automated dyn...
23296689 - Control of root meristem size by da1-related protein2 in arabidopsis.
Publication Detail:
Type:  Journal Article     Date:  2007-03-20
Journal Detail:
Title:  Cell and tissue research     Volume:  328     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-04-30     Completed Date:  2007-09-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  499-514     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine V, University of Heidelberg, Im Neuenheimer Feld 410, 69120, Heidelberg, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adherens Junctions / metabolism*,  ultrastructure
Adult
Cell Adhesion
Cell Adhesion Molecules / analysis
Cell Communication*
Cell Count
Cells, Cultured
Connexins / analysis
Cytoskeleton / metabolism
Humans
Mesenchymal Stem Cells / cytology*,  metabolism*,  ultrastructure
Microscopy, Electron
Models, Biological
Chemical
Reg. No./Substance:
0/Cell Adhesion Molecules; 0/Connexins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Follicular dendritic cells stimulated by collagen type I develop dendrites and networks in vitro.
Next Document:  Impacts of invasive plant species on riparian plant assemblages: interactions with elevated atmosphe...