Document Detail

Processing cell-seeded polyester scaffolds for histology.
MedLine Citation:
PMID:  10679693     Owner:  NLM     Status:  MEDLINE    
Biodegradable 3-dimensional scaffolds of various morphologies are currently being developed for tissue engineering. Poly(lactide-co-glycolide)s (PLGAs) of various lactide to glycolide ratios are frequently used for such applications. Tissue engineering involves an in vitro stage during which cells are seeded onto scaffolds and allowed to settle and/or grow for various time periods. To assess cell distribution and/or tissue formation throughout the scaffolds during this in vitro stage, techniques such as confocal microscopy and magnetic resonance imaging have been applied. However, such cultured scaffolds have been refractory to histological evaluation because of numerous technical difficulties. We describe a method to prepare histological sections of cell cultured PLGA scaffolds for tissue engineering. The technique involves in situ labeling of cultured scaffolds, infiltration of the scaffolds with a 10% poly(vinyl alcohol) solution under a low vacuum, and cryosectioning of samples onto acid-treated glass coverslips. Sections obtained with this technique show cell distribution and cell-tissue morphology on the pore wall structures of entire centimeter-thick scaffolds. This rapid and easy technique allows for fast evaluation of tissues grown on biodegradable scaffolds.
C E Holy; R Yakubovich
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical materials research     Volume:  50     ISSN:  0021-9304     ISO Abbreviation:  J. Biomed. Mater. Res.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-03-24     Completed Date:  2000-03-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0112726     Medline TA:  J Biomed Mater Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  276-9     Citation Subset:  IM    
Copyright Information:
Copyright 2000 John Wiley & Sons, Inc.
Institute for Biomaterials and BioMedical Engineering, Mining Building, 170 College Street, Toronto, Ontario M5S 3E3, Canada.
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MeSH Terms
Bone Substitutes*
Bone and Bones* / pathology
Histological Techniques*
Reg. No./Substance:
0/Bone Substitutes; 0/Polyesters

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