| Processing circadian data collected from the Drosophila Activity Monitoring (DAM) System. | |
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MedLine Citation:
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PMID: 21041392 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Adult behavioral assays have been used with great success in Drosophila melanogaster to identify circadian rhythm genes. In particular, the locomotor activity assay can identify altered behavior patterns over the course of several days in small populations, or even individual flies. Generally, circadian behavior is assayed during a period of 12 h light:12 h dark cycling (LD entrainment) followed by conditions of constant darkness (DD). LD activity profiles provide a qualitative image of daily activity bouts, and the data can be used to quantitatively assess the phase and/or amplitude of particular bouts. Additional activity assessments made from entrained flies that have been shifted to constant darkness can provide insight into the state of internal clocks and the ability of these clocks to drive rhythmic outputs. Typical LD DD runs assess both free-running rhythmicity and period length with χ2 periodogram (P'gram) analysis. This protocol describes the use of ClockLab (a MATLAB-based program) and the Counting Macro (an Excel-based program) to analyze circadian locomotor activity data collected using the Drosophila Activity Monitoring (DAM) System from TriKinetics. Specific procedures are described to analyze free-running rhythmicity and period length for individual flies, and assess group activity plots during both entrainment and constant conditions. |
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Authors:
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Cory Pfeiffenberger; Bridget C Lear; Kevin P Keegan; Ravi Allada |
Publication Detail:
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Type: Journal Article Date: 2010-11-01 |
Journal Detail:
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Title: Cold Spring Harbor protocols Volume: 2010 ISSN: 1559-6095 ISO Abbreviation: Cold Spring Harb Protoc Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-02 Completed Date: 2011-05-13 Revised Date: 2011-09-22 |
Medline Journal Info:
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Nlm Unique ID: 101524530 Medline TA: Cold Spring Harb Protoc Country: United States |
Other Details:
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Languages: eng Pagination: pdb.prot5519 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Circadian Rhythm* Drosophila melanogaster / physiology* Entomology / methods* Motor Activity* |
| Grant Support | |
ID/Acronym/Agency:
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R01 NS059042-04/NS/NINDS NIH HHS; R01 NS059042-05/NS/NINDS NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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