Document Detail


Processing of Chlamydia abortus polymorphic membrane protein 18D during the chlamydial developmental cycle.
MedLine Citation:
PMID:  23145118     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Chlamydia possess a unique family of autotransporter proteins known as the Polymorphic membrane proteins (Pmps). While the total number of pmp genes varies between Chlamydia species, all encode a single pmpD gene. In both Chlamydia trachomatis (C. trachomatis) and C. pneumoniae, the PmpD protein is proteolytically cleaved on the cell surface. The current study was carried out to determine the cleavage patterns of the PmpD protein in the animal pathogen C. abortus (termed Pmp18D).
METHODOLOGY/PRINCIPAL FINDINGS: Using antibodies directed against different regions of Pmp18D, proteomic techniques revealed that the mature protein was cleaved on the cell surface, resulting in a100 kDa N-terminal product and a 60 kDa carboxy-terminal protein. The N-terminal protein was further processed into 84, 76 and 73 kDa products. Clustering analysis resolved PmpD proteins into three distinct clades with C. abortus Pmp18D, being most similar to those originating from C. psittaci, C. felis and C. caviae.
CONCLUSIONS/SIGNIFICANCE: This study indicates that C. abortus Pmp18D is proteolytically processed at the cell surface similar to the proteins of C. trachomatis and C. pneumoniae. However, patterns of cleavage are species-specific, with low sequence conservation of PmpD across the genus. The absence of conserved domains indicates that the function of the PmpD molecule in chlamydia remains to be elucidated.
Authors:
Nick M Wheelhouse; Michelle Sait; Kevin Aitchison; Morag Livingstone; Frank Wright; Kevin McLean; Neil F Inglis; David G E Smith; David Longbottom
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-08
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-11-12     Completed Date:  2013-05-23     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e49190     Citation Subset:  IM    
Affiliation:
Moredun Research Institute, Edinburgh, Midlothian, United Kingdom. nick.wheelhouse@moredun.ac.uk
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins* / genetics,  metabolism
Chlamydia Infections / genetics,  metabolism,  microbiology
Chlamydia trachomatis* / genetics,  metabolism
Gene Expression Regulation, Bacterial
Humans
Membrane Proteins* / genetics,  metabolism
Phylogeny
Proteomics / methods
Sequence Homology, Amino Acid
Grant Support
ID/Acronym/Agency:
BB/E018939/1//Biotechnology and Biological Sciences Research Council
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Membrane Proteins; 0/polymorphic membrane protein D, Chlamydia trachomatis
Comments/Corrections

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