| Process-scale disruption of microorganisms. | |
| | |
MedLine Citation:
|
PMID: 14536098 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
|
Common hosts for the large-scale manufacture of biological products, such as Escherichia coli and Saccharomyces cerevisiae, do not excrete products to the medium. Effective techniques for cell disruption are therefore required. These include physical, chemical, enzymatic and mechanical methods. Mechanical methods such as bead milling, high-pressure homogenization, and microfluidization are preferred. However, gentler, specific methods are receiving increasing attention particularly when used in combination to synergistically exploit their different specificities. Benefits can also be derived by integrating product release and recovery. In all cases it is essential to consider the interaction of the disruption operation with downstream units and to clearly demonstrate the cost benefits of alternative strategies. |
| | |
Authors:
|
A P Middelberg |
Related Documents
:
|
6351148 - Attention and autonomic self-regulation. 18199918 - Prevalence and correlates of multiple organ damage in a never-treated hypertensive popu... 7318338 - Association between the c3f-gene and essential hypertension. 16240528 - Testing method of plus and minus gz tolerance at czech air force pilots. 16505658 - Vasopressors and intestinal mucosal perfusion after cardiac surgery: norepinephrine vs.... 19207698 - Differences in ano-neorectal physiology of ileoanal and coloanal reconstructions for re... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biotechnology advances Volume: 13 ISSN: 0734-9750 ISO Abbreviation: Biotechnol. Adv. Publication Date: 1995 |
Date Detail:
|
Created Date: 2003-10-10 Completed Date: 2003-12-23 Revised Date: 2006-07-07 |
Medline Journal Info:
|
Nlm Unique ID: 8403708 Medline TA: Biotechnol Adv Country: England |
Other Details:
|
Languages: eng Pagination: 491-551 Citation Subset: - |
Affiliation:
|
Co-operative Research Centre for Tissue Growth and Repair, Department of Chemical Engineering, The University of Adelaide, SA 5005, Australia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The impact of biotechnology on hyphomycetous fungal insect biocontrol agents.
Next Document: Factors affecting the genetic engineering of plants by microprojectile bombardment.